Chemical Modifications of Globular Proteins Phototriggered by an Endogenous Photosensitizer

The main goal of the present work was to investigate the damages photoinduced by pterin (Ptr), an endogenous photosensitizer present in human skin under pathological conditions, on a globular protein such as ubiquitin (Ub). A particular attention has been paid on the formation of covalent adducts be...

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Detalles Bibliográficos
Autores principales: Reid, Lara Olivia, Dántola, María Laura, Petroselli, Gabriela, Erra Balsells, Rosa, Miranda, Miguel A., Lhiaubet Vallet, Virginie, Thomas, Andrés Héctor
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Química
Mass spectrometry
Adducts
Peptides and proteins
Fluorescence
Irradiation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125040
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125040
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Mass spectrometry
Adducts
Peptides and proteins
Fluorescence
Irradiation
spellingShingle Química
Mass spectrometry
Adducts
Peptides and proteins
Fluorescence
Irradiation
Reid, Lara Olivia
Dántola, María Laura
Petroselli, Gabriela
Erra Balsells, Rosa
Miranda, Miguel A.
Lhiaubet Vallet, Virginie
Thomas, Andrés Héctor
Chemical Modifications of Globular Proteins Phototriggered by an Endogenous Photosensitizer
topic_facet Química
Mass spectrometry
Adducts
Peptides and proteins
Fluorescence
Irradiation
description The main goal of the present work was to investigate the damages photoinduced by pterin (Ptr), an endogenous photosensitizer present in human skin under pathological conditions, on a globular protein such as ubiquitin (Ub). A particular attention has been paid on the formation of covalent adducts between Ptr and the protein that can behave as photoantigen and provoke an immune system response. Here, a multifaceted approach including UV/visible spectrophotometry, fluorescence spectroscopy, electrophoresis, size exclusion chromatography and mass spectrometry is used to establish the Ub changes triggered by UVA irradiation in the presence of Ptr. Under anaerobic conditions, the only reaction corresponds to the formation of a covalently bound Ptr-Ub adduct that retains the spectroscopic properties of the free photosensitizer. A more complex scheme is observed in air-equilibrated solutions with the occurrence of three different processes ie. formation of a Ptr-Ub adduct, dimerization and fragmentation of the protein.
format Articulo
Preprint
author Reid, Lara Olivia
Dántola, María Laura
Petroselli, Gabriela
Erra Balsells, Rosa
Miranda, Miguel A.
Lhiaubet Vallet, Virginie
Thomas, Andrés Héctor
author_facet Reid, Lara Olivia
Dántola, María Laura
Petroselli, Gabriela
Erra Balsells, Rosa
Miranda, Miguel A.
Lhiaubet Vallet, Virginie
Thomas, Andrés Héctor
author_sort Reid, Lara Olivia
title Chemical Modifications of Globular Proteins Phototriggered by an Endogenous Photosensitizer
title_short Chemical Modifications of Globular Proteins Phototriggered by an Endogenous Photosensitizer
title_full Chemical Modifications of Globular Proteins Phototriggered by an Endogenous Photosensitizer
title_fullStr Chemical Modifications of Globular Proteins Phototriggered by an Endogenous Photosensitizer
title_full_unstemmed Chemical Modifications of Globular Proteins Phototriggered by an Endogenous Photosensitizer
title_sort chemical modifications of globular proteins phototriggered by an endogenous photosensitizer
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/125040
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