Genomic Diversity in a Population of Spodoptera frugiperda Nucleopolyhedrovirus

Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. However, little is known regard...

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Detalles Bibliográficos
Autores principales: Masson, Tomás, Fabre, María Laura, Pidre, Matías Luis, Niz, José María, Berretta, Marcelo Facundo, Romanowski, Víctor, Ferrelli, María Leticia
Formato: Articulo Preprint
Lenguaje:Español
Publicado: 2020
Materias:
Bioquímica
Ciencias Exactas
baculovirus
genomics
diversity
evolution
SfMNPV
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124946
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124946
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Bioquímica
Ciencias Exactas
baculovirus
genomics
diversity
evolution
SfMNPV
spellingShingle Bioquímica
Ciencias Exactas
baculovirus
genomics
diversity
evolution
SfMNPV
Masson, Tomás
Fabre, María Laura
Pidre, Matías Luis
Niz, José María
Berretta, Marcelo Facundo
Romanowski, Víctor
Ferrelli, María Leticia
Genomic Diversity in a Population of Spodoptera frugiperda Nucleopolyhedrovirus
topic_facet Bioquímica
Ciencias Exactas
baculovirus
genomics
diversity
evolution
SfMNPV
description Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. However, little is known regarding the genomic diversity present inside SfMNPV isolates and how it shapes the interactions between virus and host. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent.
format Articulo
Preprint
author Masson, Tomás
Fabre, María Laura
Pidre, Matías Luis
Niz, José María
Berretta, Marcelo Facundo
Romanowski, Víctor
Ferrelli, María Leticia
author_facet Masson, Tomás
Fabre, María Laura
Pidre, Matías Luis
Niz, José María
Berretta, Marcelo Facundo
Romanowski, Víctor
Ferrelli, María Leticia
author_sort Masson, Tomás
title Genomic Diversity in a Population of Spodoptera frugiperda Nucleopolyhedrovirus
title_short Genomic Diversity in a Population of Spodoptera frugiperda Nucleopolyhedrovirus
title_full Genomic Diversity in a Population of Spodoptera frugiperda Nucleopolyhedrovirus
title_fullStr Genomic Diversity in a Population of Spodoptera frugiperda Nucleopolyhedrovirus
title_full_unstemmed Genomic Diversity in a Population of Spodoptera frugiperda Nucleopolyhedrovirus
title_sort genomic diversity in a population of spodoptera frugiperda nucleopolyhedrovirus
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/124946
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