Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in <i>Medicago truncatula</i>

Auxin Response Factors (ARFs) constitute a large family of transcription factors that mediate auxin-regulated developmental programs in plants. ARF2, ARF3, and ARF4 are post-transcriptionally regulated by the microRNA390 (miR390)/trans-acting small interference RNA 3 (TAS3) module through the action...

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Autores principales: Kirolinko, Cristina, Hobecker, Karen Vanesa, Wen, Jiangqi, Mysore, Kirankumar S., Niebel, Andreas, Blanco, Flavio Antonio, Zanetti, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Ciencias Exactas
Biología
auxin response factors
legumes
Nod Factor
miR390
root architecture
symbiosis
tasiARFs
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124866
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124866
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
auxin response factors
legumes
Nod Factor
miR390
miR390
root architecture
symbiosis
tasiARFs
spellingShingle Ciencias Exactas
Biología
auxin response factors
legumes
Nod Factor
miR390
miR390
root architecture
symbiosis
tasiARFs
Kirolinko, Cristina
Hobecker, Karen Vanesa
Wen, Jiangqi
Mysore, Kirankumar S.
Niebel, Andreas
Blanco, Flavio Antonio
Zanetti, María Eugenia
Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in <i>Medicago truncatula</i>
topic_facet Ciencias Exactas
Biología
auxin response factors
legumes
Nod Factor
miR390
miR390
root architecture
symbiosis
tasiARFs
description Auxin Response Factors (ARFs) constitute a large family of transcription factors that mediate auxin-regulated developmental programs in plants. ARF2, ARF3, and ARF4 are post-transcriptionally regulated by the microRNA390 (miR390)/trans-acting small interference RNA 3 (TAS3) module through the action of TAS3-derived trans - acting small interfering RNAs (ta-siRNA). We have previously reported that constitutive activation of the miR390/TAS3 pathway promotes elongation of lateral roots but impairs nodule organogenesis and infection by rhizobia during the nitrogen-fixing symbiosis established between <i>Medicago truncatula</i> and its partner <i>Sinorhizobium meliloti</i>. However, the involvement of the targets of the miR390/TAS3 pathway, i.e., MtARF2, MtARF3, MtARF4a, and MtARF4b, in root development and establishment of the nitrogen-fixing symbiosis remained unexplored. Here, promoter:reporter fusions showed that expression of both MtARF3 and MtARF4a was associated with lateral root development; however, only the MtARF4a promoter was active in developing nodules. In addition, up-regulation of MtARF2, MtARF3, and MtARF4a/b in response to rhizobia depends on Nod Factor perception. We provide evidence that simultaneous knockdown of MtARF2, MtARF3, MtARF4a, and MtARF4b or mutation in MtARF4a impaired nodule formation, and reduced initiation and progression of infection events. Silencing of MtARF2, MtARF3, MtARF4a, and MtARF4b altered mRNA levels of the early nodulation gene nodulation signaling pathway 2 (MtNSP2). In addition, roots with reduced levels of MtARF2, MtARF3, MtARF4a, and MtARF4b, as well as arf4a mutant plants exhibited altered root architecture, causing a reduction in primary and lateral root length, but increasing lateral root density. Taken together, our results suggest that these ARF members are common key players of the morphogenetic programs that control root development and the formation of nitrogen-fixing nodules.
format Articulo
Articulo
author Kirolinko, Cristina
Hobecker, Karen Vanesa
Wen, Jiangqi
Mysore, Kirankumar S.
Niebel, Andreas
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_facet Kirolinko, Cristina
Hobecker, Karen Vanesa
Wen, Jiangqi
Mysore, Kirankumar S.
Niebel, Andreas
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_sort Kirolinko, Cristina
title Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in <i>Medicago truncatula</i>
title_short Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in <i>Medicago truncatula</i>
title_full Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in <i>Medicago truncatula</i>
title_fullStr Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in <i>Medicago truncatula</i>
title_full_unstemmed Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in <i>Medicago truncatula</i>
title_sort auxin response factor 2 (arf2), arf3, and arf4 mediate both lateral root and nitrogen fixing nodule development in <i>medicago truncatula</i>
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/124866
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