Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs

Translational control is a widespread mechanism that allows the cell to rapidly modulate gene expression in order to provide flexibility and adaptability to eukaryotic organisms. We applied translating ribosome affinity purification combined with RNA sequencing to characterize translational regulati...

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Autores principales: Traubenik, Laura Soledad, Reynoso, Mauricio Alberto, Hobecker, Karen Vanesa, Lancia, Marcos, Hummel, Maureen, Rosen, Benjamin D., Town, Christopher D., Bailey Serres, Julia, Blanco, Flavio Antonio, Zanetti, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
Biología
Cell biology
Root Cells
Nitrogen-Fixing Symbiosis
Coding and Noncoding RNAs
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124792
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124792
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Cell biology
Root Cells
Nitrogen-Fixing Symbiosis
Coding and Noncoding RNAs
spellingShingle Ciencias Exactas
Biología
Cell biology
Root Cells
Nitrogen-Fixing Symbiosis
Coding and Noncoding RNAs
Traubenik, Laura Soledad
Reynoso, Mauricio Alberto
Hobecker, Karen Vanesa
Lancia, Marcos
Hummel, Maureen
Rosen, Benjamin D.
Town, Christopher D.
Bailey Serres, Julia
Blanco, Flavio Antonio
Zanetti, María Eugenia
Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs
topic_facet Ciencias Exactas
Biología
Cell biology
Root Cells
Nitrogen-Fixing Symbiosis
Coding and Noncoding RNAs
description Translational control is a widespread mechanism that allows the cell to rapidly modulate gene expression in order to provide flexibility and adaptability to eukaryotic organisms. We applied translating ribosome affinity purification combined with RNA sequencing to characterize translational regulation of mRNAs at early stages of the nitrogen-fixing symbiosis established between <i>Medicago truncatula</i> and <i>Sinorhizobium meliloti</i>. Our analysis revealed a poor correlation between transcriptional and translational changes and identified hundreds of regulated protein-coding and long noncoding RNAs (lncRNAs), some of which are regulated in specific cell types. We demonstrated that a short variant of the lncRNA <i>Trans-acting small interference RNA3</i> (<i>TAS3</i>) increased its association to the translational machinery in response to rhizobia. Functional analysis revealed that this short variant of <i>TAS3</i> might act as a target mimic that captures microRNA390, contributing to reduce trans acting small interference Auxin Response Factor production and modulating nodule formation and rhizobial infection. The analysis of alternative transcript variants identified a translationally upregulated mRNA encoding subunit 3 of the SUPERKILLER complex (SKI3), which participates in mRNA decay. Knockdown of <i>SKI3</i> decreased nodule initiation and development, as well as the survival of bacteria within nodules. Our results highlight the importance of translational control and mRNA decay pathways for the successful establishment of the nitrogen-fixing symbiosis.
format Articulo
Articulo
author Traubenik, Laura Soledad
Reynoso, Mauricio Alberto
Hobecker, Karen Vanesa
Lancia, Marcos
Hummel, Maureen
Rosen, Benjamin D.
Town, Christopher D.
Bailey Serres, Julia
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_facet Traubenik, Laura Soledad
Reynoso, Mauricio Alberto
Hobecker, Karen Vanesa
Lancia, Marcos
Hummel, Maureen
Rosen, Benjamin D.
Town, Christopher D.
Bailey Serres, Julia
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_sort Traubenik, Laura Soledad
title Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs
title_short Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs
title_full Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs
title_fullStr Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs
title_full_unstemmed Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs
title_sort reprogramming of root cells during nitrogen-fixing symbiosis involves dynamic polysome association of coding and noncoding rnas
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/124792
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