A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production

<i>Fasciola hepatica</i> is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the d...

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Autores principales: Silvane, Leonardo Micael, Celias, Daiana Pamela, Romagnoli, Pablo Alberto, Maletto, Belkys Angélica, Sánchez Vallecillo, María Fernanda, Chiapello, Laura Silvina, Palma, Santiago Daniel, Allemandi, Daniel Alberto, Sanabria, Rodrigo Eduardo Fabrizio, Pruzzo, César Iván, Motrán, Claudia Cristina, Cervi, Laura
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Médicas
Ciencias Exactas
Veterinaria
Th17-dependent protection
nanostructure
ascorbyl palmitate
kunitz type molecule
vaccine
Fasciola hepatica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124518
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124518
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Ciencias Exactas
Veterinaria
Th17-dependent protection
nanostructure
ascorbyl palmitate
kunitz type molecule
vaccine
Fasciola hepatica
spellingShingle Ciencias Médicas
Ciencias Exactas
Veterinaria
Th17-dependent protection
nanostructure
ascorbyl palmitate
kunitz type molecule
vaccine
Fasciola hepatica
Silvane, Leonardo Micael
Celias, Daiana Pamela
Romagnoli, Pablo Alberto
Maletto, Belkys Angélica
Sánchez Vallecillo, María Fernanda
Chiapello, Laura Silvina
Palma, Santiago Daniel
Allemandi, Daniel Alberto
Sanabria, Rodrigo Eduardo Fabrizio
Pruzzo, César Iván
Motrán, Claudia Cristina
Cervi, Laura
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production
topic_facet Ciencias Médicas
Ciencias Exactas
Veterinaria
Th17-dependent protection
nanostructure
ascorbyl palmitate
kunitz type molecule
vaccine
Fasciola hepatica
description <i>Fasciola hepatica</i> is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the development of parasite resistance and, consequently, the limitation of its effectiveness. Hence, vaccination appears as an attractive option to develop. In this work, we evaluated the potential of <i>F. hepatica</i> Kunitz-type molecule (FhKTM) as an antigen formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) during an experimental model of fasciolosis in mice, and we further dissected the immune response associated with host protection. Our results showed that immunization of mice with FhKTM/CpG-ODN/Coa-ASC16 induces protection against <i>F. hepatica</i> challenge by preventing liver damage and improving survival after <i>F. hepatica</i> infection. FhKTM/CpG-ODN/Coa-ASC16-immunized mice elicited potent IFN-γ and IL-17A with high levels of antigen-specific IgG1, IgG2a, and IgA serum antibodies. Strikingly, IL-17A blockade during infection decreased IgG2a and IgA antibody levels as well as IFN-γ production, leading to an increase in mortality of vaccinated mice. The present study highlights the potential of a new vaccine formulation to improve control and help the eradication of <i>F. hepatica</i> infection, with potential applications for natural hosts such as cattle and sheep.
format Articulo
Articulo
author Silvane, Leonardo Micael
Celias, Daiana Pamela
Romagnoli, Pablo Alberto
Maletto, Belkys Angélica
Sánchez Vallecillo, María Fernanda
Chiapello, Laura Silvina
Palma, Santiago Daniel
Allemandi, Daniel Alberto
Sanabria, Rodrigo Eduardo Fabrizio
Pruzzo, César Iván
Motrán, Claudia Cristina
Cervi, Laura
author_facet Silvane, Leonardo Micael
Celias, Daiana Pamela
Romagnoli, Pablo Alberto
Maletto, Belkys Angélica
Sánchez Vallecillo, María Fernanda
Chiapello, Laura Silvina
Palma, Santiago Daniel
Allemandi, Daniel Alberto
Sanabria, Rodrigo Eduardo Fabrizio
Pruzzo, César Iván
Motrán, Claudia Cristina
Cervi, Laura
author_sort Silvane, Leonardo Micael
title A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production
title_short A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production
title_full A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production
title_fullStr A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production
title_full_unstemmed A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production
title_sort vaccine based on kunitz-type molecule confers protection against <i>fasciola hepatica</i> challenge by inducing ifn-γ and antibody immune responses through il-17a production
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/124518
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bdutipo_str Repositorios
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