The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of Lymnaea columella, L. viatrix, and L. diaphana (Mollusca: Lymnaeidae)

The specific identification of Lymnaeid snails is based on a comparison of morphological characters of the shell, radula, renal and reproductive organs. However, the identification is complicated by dissection process, intra and interspecific similarity and variability of morphological characters. I...

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Autores principales:	Santos Carvalho, Omar dos, Marques Cardoso, Paula Cristina, Lira, Pollanah M., Rumi Macchi Zubiaurre, Alejandra, Roche, María Andrea de las Mercedes, Berne, Elizabeth, Müller, Gertrud, Caldeira, Roberta Lima
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2004
Materias:	Ciencias Naturales Lymnaea columella Lymnaea viatrix Lymnaea diaphana polymerase chain reaction and restriction fragment length polymorphism first and second internal transcribed spacers of the RNA ribosomal gene subunit 16S of mitochondrial RNA ribosomal gene
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/123935
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-123935
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Lymnaea columella Lymnaea viatrix Lymnaea diaphana polymerase chain reaction and restriction fragment length polymorphism first and second internal transcribed spacers of the RNA ribosomal gene subunit 16S of mitochondrial RNA ribosomal gene
spellingShingle	Ciencias Naturales Lymnaea columella Lymnaea viatrix Lymnaea diaphana polymerase chain reaction and restriction fragment length polymorphism first and second internal transcribed spacers of the RNA ribosomal gene subunit 16S of mitochondrial RNA ribosomal gene Santos Carvalho, Omar dos Marques Cardoso, Paula Cristina Lira, Pollanah M. Rumi Macchi Zubiaurre, Alejandra Roche, María Andrea de las Mercedes Berne, Elizabeth Müller, Gertrud Caldeira, Roberta Lima The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> (Mollusca: Lymnaeidae)
topic_facet	Ciencias Naturales Lymnaea columella Lymnaea viatrix Lymnaea diaphana polymerase chain reaction and restriction fragment length polymorphism first and second internal transcribed spacers of the RNA ribosomal gene subunit 16S of mitochondrial RNA ribosomal gene
description	The specific identification of Lymnaeid snails is based on a comparison of morphological characters of the shell, radula, renal and reproductive organs. However, the identification is complicated by dissection process, intra and interspecific similarity and variability of morphological characters. In the present study, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) techniques targeted to the first and second internal transcribed spacers (ITS1 and ITS2) rDNA and to the mitochondrial 16S ribosomal gene (16S rDNAmt) were used to differentiate the species <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> from some localities of Brazil, Argentina, and Uruguay as well as to verify whether the molecular results corroborates the classical morphological method.PCR-RFLP analysis of the ITS1, ITS2, and 16S using 12 restriction enzymes revealed characteristic patterns for <i>L. columella</i> and <i>L. diaphana</i> which were concordant with the classical morphology. On the other hand, for <i>L. viatrix</i> populations a number of 1 to 6 profiles were generated while morphology provided the species pattern results.
format	Articulo Articulo
author	Santos Carvalho, Omar dos Marques Cardoso, Paula Cristina Lira, Pollanah M. Rumi Macchi Zubiaurre, Alejandra Roche, María Andrea de las Mercedes Berne, Elizabeth Müller, Gertrud Caldeira, Roberta Lima
author_facet	Santos Carvalho, Omar dos Marques Cardoso, Paula Cristina Lira, Pollanah M. Rumi Macchi Zubiaurre, Alejandra Roche, María Andrea de las Mercedes Berne, Elizabeth Müller, Gertrud Caldeira, Roberta Lima
author_sort	Santos Carvalho, Omar dos
title	The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> (Mollusca: Lymnaeidae)
title_short	The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> (Mollusca: Lymnaeidae)
title_full	The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> (Mollusca: Lymnaeidae)
title_fullStr	The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> (Mollusca: Lymnaeidae)
title_full_unstemmed	The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> (Mollusca: Lymnaeidae)
title_sort	use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>lymnaea columella</i>, <i>l. viatrix</i>, and <i>l. diaphana</i> (mollusca: lymnaeidae)
publishDate	2004
url	http://sedici.unlp.edu.ar/handle/10915/123935
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The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of <i>Lymnaea columella</i>, <i>L. viatrix</i>, and <i>L. diaphana</i> (Mollusca: Lymnaeidae)

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