Gene discovery through genomic sequencing of Brucella abortus.

Brucella abortus is the etiological agent of brucellosis, a disease that affects bovines and human. We generated DNA random sequences from the genome of B. abortus strain 2308 in order to characterize molecular targets that might be useful for developing immunological or chemotherapeutic strategies...

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Autores principales: Sánchez, Daniel O., Zandomeni, Rubén O., Cravero, Silvio, Verdun, Ramiro E., Pierrou, Ester, Faccio, Paula, Diaz, Gabriela, Lanzavecchia, Silvia, Agüero, Fernán, Frasch, Alberto C.C., Andersson, Siv G. E., Rossetti, Osvaldo, Grau, Oscar, Ugalde, Rodolfo A
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123814
Aporte de:
id I19-R120-10915-123814
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Biología
Brucella abortus
Genes
Pathogens
spellingShingle Ciencias Naturales
Biología
Brucella abortus
Genes
Pathogens
Sánchez, Daniel O.
Zandomeni, Rubén O.
Cravero, Silvio
Verdun, Ramiro E.
Pierrou, Ester
Faccio, Paula
Diaz, Gabriela
Lanzavecchia, Silvia
Agüero, Fernán
Frasch, Alberto C.C.
Andersson, Siv G. E.
Rossetti, Osvaldo
Grau, Oscar
Ugalde, Rodolfo A
Gene discovery through genomic sequencing of Brucella abortus.
topic_facet Ciencias Naturales
Biología
Brucella abortus
Genes
Pathogens
description Brucella abortus is the etiological agent of brucellosis, a disease that affects bovines and human. We generated DNA random sequences from the genome of B. abortus strain 2308 in order to characterize molecular targets that might be useful for developing immunological or chemotherapeutic strategies against this pathogen. The partial sequencing of 1,899 clones allowed the identification of 1,199 genomic sequence surveys (GSSs) with high homology (BLAST expect value < 10(-5)) to sequences deposited in the GenBank databases. Among them, 925 represent putative novel genes for the Brucella genus. Out of 925 nonredundant GSSs, 470 were classified in 15 categories based on cellular function. Seven hundred GSSs showed no significant database matches and remain available for further studies in order to identify their function. A high number of GSSs with homology to Agrobacterium tumefaciens and Rhizobium meliloti proteins were observed, thus confirming their close phylogenetic relationship. Among them, several GSSs showed high similarity with genes related to nodule nitrogen fixation, synthesis of nod factors, nodulation protein symbiotic plasmid, and nodule bacteroid differentiation. We have also identified several B. abortus homologs of virulence and pathogenesis genes from other pathogens, including a homolog to both the Shda gene from Salmonella enterica serovar Typhimurium and the AidA-1 gene from Escherichia coli. Other GSSs displayed significant homologies to genes encoding components of the type III and type IV secretion machineries, suggesting that Brucella might also have an active type III secretion machinery.
format Articulo
Articulo
author Sánchez, Daniel O.
Zandomeni, Rubén O.
Cravero, Silvio
Verdun, Ramiro E.
Pierrou, Ester
Faccio, Paula
Diaz, Gabriela
Lanzavecchia, Silvia
Agüero, Fernán
Frasch, Alberto C.C.
Andersson, Siv G. E.
Rossetti, Osvaldo
Grau, Oscar
Ugalde, Rodolfo A
author_facet Sánchez, Daniel O.
Zandomeni, Rubén O.
Cravero, Silvio
Verdun, Ramiro E.
Pierrou, Ester
Faccio, Paula
Diaz, Gabriela
Lanzavecchia, Silvia
Agüero, Fernán
Frasch, Alberto C.C.
Andersson, Siv G. E.
Rossetti, Osvaldo
Grau, Oscar
Ugalde, Rodolfo A
author_sort Sánchez, Daniel O.
title Gene discovery through genomic sequencing of Brucella abortus.
title_short Gene discovery through genomic sequencing of Brucella abortus.
title_full Gene discovery through genomic sequencing of Brucella abortus.
title_fullStr Gene discovery through genomic sequencing of Brucella abortus.
title_full_unstemmed Gene discovery through genomic sequencing of Brucella abortus.
title_sort gene discovery through genomic sequencing of brucella abortus.
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/123814
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