In Vitro Antagonistic Activity of <i>Trichoderma harzianum</i> against <i>Fusarium sudanense</i> Causing Seedling Blight and Seed Rot on Wheat

<i>Fusarium sudanense</i> is a novel fungus recently isolated from asymptomatic samples of wheat grains in Argentina. The fungus caused symptoms of seedling blight and seed rot on wheat after artificial inoculations. It is known that the production of mycotoxins by pathogens belonging to...

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Detalles Bibliográficos
Autores principales: Larrán, Silvina, Santamarina Siurana, M. Pilar, Caselles, Josefa Roselló, Simón, María Rosa, Perelló, Analía Edith
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Ciencias Naturales
Fungus
Mycotoxin production
Dominance index
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123710
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123710
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Naturales
Fungus
Mycotoxin production
Dominance index
spellingShingle Biología
Ciencias Naturales
Fungus
Mycotoxin production
Dominance index
Larrán, Silvina
Santamarina Siurana, M. Pilar
Caselles, Josefa Roselló
Simón, María Rosa
Perelló, Analía Edith
In Vitro Antagonistic Activity of <i>Trichoderma harzianum</i> against <i>Fusarium sudanense</i> Causing Seedling Blight and Seed Rot on Wheat
topic_facet Biología
Ciencias Naturales
Fungus
Mycotoxin production
Dominance index
description <i>Fusarium sudanense</i> is a novel fungus recently isolated from asymptomatic samples of wheat grains in Argentina. The fungus caused symptoms of seedling blight and seed rot on wheat after artificial inoculations. It is known that the production of mycotoxins by pathogens belonging to the <i>Fusarium genus</i> is harmful to human and animal health. Moreover, the warm and humid conditions that are favorable for growth and mycotoxin production of these species put the Argentinian wheat production area at a high risk of mycotoxin contamination with this novel pathogen. The aim of this work was to evaluate the antagonistic effect of <i>Trichoderma harzianum</i> against <i>F. sudanense</i> under in vitro tests at different environmental conditions. Fungi were screened in dual culture at different water activities (αw) (0.995, 0.98, 0.95, and 0.90) and temperatures (25 and 15 °C). The growth rate of the fungi, interaction types, and dominance index were evaluated. Also, the interaction between <i>T. harzianum</i> and <i>F. sudanense</i> was examined by light and cryo-scanning microscopy. <i>T. harzianum</i> suppressed the growth of <i>F. sudanense</i> at 0.995, 0.98, and 0.95 αw at 25 °C and 0.995 and 0.98 αw at 15 °C. Macroscopic study revealed different interaction types between <i>F. sudanense</i> and <i>T. harzianum</i> on dual culture. Dominance on contact where the colonies of <i>T. harzianum</i> overgrew the pathogen was the most common interaction type determined. The competitive capacity of <i>T. harzianum</i> was diminished by decreasing the temperature and αw. At 0.95 αw and 15 °C, both fungi grew slowly, and interaction type "A" was assigned. Microscopic analysis from the interaction zone of dual cultures revealed an attachment of <i>T. harzianum</i> to the <i>F. sudanense hyphae</i>, penetration with or without formation of appressorium-like structures, coiling, plasmolysis, and a veil formation. According to our results, <i>T. harzianum</i> demonstrated capability to antagonize <i>F. sudanense</i> and could be a promising biocontrol agent.
format Articulo
Articulo
author Larrán, Silvina
Santamarina Siurana, M. Pilar
Caselles, Josefa Roselló
Simón, María Rosa
Perelló, Analía Edith
author_facet Larrán, Silvina
Santamarina Siurana, M. Pilar
Caselles, Josefa Roselló
Simón, María Rosa
Perelló, Analía Edith
author_sort Larrán, Silvina
title In Vitro Antagonistic Activity of <i>Trichoderma harzianum</i> against <i>Fusarium sudanense</i> Causing Seedling Blight and Seed Rot on Wheat
title_short In Vitro Antagonistic Activity of <i>Trichoderma harzianum</i> against <i>Fusarium sudanense</i> Causing Seedling Blight and Seed Rot on Wheat
title_full In Vitro Antagonistic Activity of <i>Trichoderma harzianum</i> against <i>Fusarium sudanense</i> Causing Seedling Blight and Seed Rot on Wheat
title_fullStr In Vitro Antagonistic Activity of <i>Trichoderma harzianum</i> against <i>Fusarium sudanense</i> Causing Seedling Blight and Seed Rot on Wheat
title_full_unstemmed In Vitro Antagonistic Activity of <i>Trichoderma harzianum</i> against <i>Fusarium sudanense</i> Causing Seedling Blight and Seed Rot on Wheat
title_sort in vitro antagonistic activity of <i>trichoderma harzianum</i> against <i>fusarium sudanense</i> causing seedling blight and seed rot on wheat
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/123710
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