Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice

Intestinal-fatty acid binding protein (IFABP; FABP2) is a 15-kDa intracellular protein abundantly present in the cytosol of the small intestinal (SI) enterocyte. High-fat (HF) feeding of IFABP-/- mice resulted in reduced weight gain and fat mass relative to wild-type (WT) mice. Here, we examined int...

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Autores principales: Lackey, Atreju I., Chen, Tina, Zhou, Yin X, Bottasso Arias, Natalia María, Doran, Justine M., Zacharisen, Sophia M., Gajda, Angela M., Jonsson, William O., Córsico, Betina, Anthony, Tracy G., Joseph, Laurie B., Storch, Judith
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Bioquímica
Ciencias Exactas
IFABP
Intestine
Lipid
Morphology
Nutrition
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123452
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123452
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Ciencias Exactas
IFABP
Intestine
Lipid
Morphology
Nutrition
spellingShingle Bioquímica
Ciencias Exactas
IFABP
Intestine
Lipid
Morphology
Nutrition
Lackey, Atreju I.
Chen, Tina
Zhou, Yin X
Bottasso Arias, Natalia María
Doran, Justine M.
Zacharisen, Sophia M.
Gajda, Angela M.
Jonsson, William O.
Córsico, Betina
Anthony, Tracy G.
Joseph, Laurie B.
Storch, Judith
Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
topic_facet Bioquímica
Ciencias Exactas
IFABP
Intestine
Lipid
Morphology
Nutrition
description Intestinal-fatty acid binding protein (IFABP; FABP2) is a 15-kDa intracellular protein abundantly present in the cytosol of the small intestinal (SI) enterocyte. High-fat (HF) feeding of IFABP-/- mice resulted in reduced weight gain and fat mass relative to wild-type (WT) mice. Here, we examined intestinal properties that may underlie the observed lean phenotype of high fat-fed IFABP-/- mice. No alterations in fecal lipid content were found, suggesting that the IFABP-/- mice are not malabsorbing dietary fat. However, the total excreted fecal mass, normalized to food intake, was increased for the IFABP-/- mice relative to WT mice. Moreover, intestinal transit time was more rapid in the IFABP-/- mice. IFABP-/- mice displayed a shortened average villus length, a thinner muscularis layer, reduced goblet cell density, and reduced Paneth cell abundance. The number of proliferating cells in the crypts of IFABP-/- mice did not differ from that of WT mice, suggesting that the blunt villi phenotype is not due to alterations in proliferation. IFABP-/- mice were observed to have altered expression of genes and proteins related to intestinal structure, while immunohistochemical analyses revealed increased staining for markers of inflammation. Taken together, these studies indicate that the ablation of IFABP, coupled with high-fat feeding, leads to changes in gut motility and morphology, which likely contribute to the relatively leaner phenotype occurring at the whole-body level. Thus, IFABP is likely involved in dietary lipid sensing and signaling, influencing intestinal motility, intestinal structure, and nutrient absorption, thereby impacting systemic energy metabolism.
format Articulo
Articulo
author Lackey, Atreju I.
Chen, Tina
Zhou, Yin X
Bottasso Arias, Natalia María
Doran, Justine M.
Zacharisen, Sophia M.
Gajda, Angela M.
Jonsson, William O.
Córsico, Betina
Anthony, Tracy G.
Joseph, Laurie B.
Storch, Judith
author_facet Lackey, Atreju I.
Chen, Tina
Zhou, Yin X
Bottasso Arias, Natalia María
Doran, Justine M.
Zacharisen, Sophia M.
Gajda, Angela M.
Jonsson, William O.
Córsico, Betina
Anthony, Tracy G.
Joseph, Laurie B.
Storch, Judith
author_sort Lackey, Atreju I.
title Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_short Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_full Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_fullStr Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_full_unstemmed Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_sort mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (ifabp) null mice
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/123452
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