Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by 51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25 ◦C. The results showed that decomposition products are orthovanadate [H2VO4]−...

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Detalles Bibliográficos
Autores principales: Silva Nolasco, Aniela M., Camacho, Luz, Saavedra Díaz, Rafael Omar, Hernández Abreu, Oswaldo, León, Ignacio Esteban, Sánchez Lombardo, Irma
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Química
Polyoxometalates
Decavanadate
Cytotoxicity
Insulin-like activity
Diabetes therapy
Vanadium biochemistry
Vanadium speciation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/119728
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-119728
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Polyoxometalates
Decavanadate
Cytotoxicity
Insulin-like activity
Diabetes therapy
Vanadium biochemistry
Vanadium speciation
spellingShingle Química
Polyoxometalates
Decavanadate
Cytotoxicity
Insulin-like activity
Diabetes therapy
Vanadium biochemistry
Vanadium speciation
Silva Nolasco, Aniela M.
Camacho, Luz
Saavedra Díaz, Rafael Omar
Hernández Abreu, Oswaldo
León, Ignacio Esteban
Sánchez Lombardo, Irma
Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity
topic_facet Química
Polyoxometalates
Decavanadate
Cytotoxicity
Insulin-like activity
Diabetes therapy
Vanadium biochemistry
Vanadium speciation
description The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by 51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25 ◦C. The results showed that decomposition products are orthovanadate [H2VO4]− (V1) and metavanadate species like [H2V2O7]2− (V2), [V4O12]4− (V4) and [V5O15]5− (V5) for both compounds. The calculated half-life times of the decomposition reaction were 9 and 11 h for NaDeca and MetfDeca, respectively, at 1 mM concentration. The hydrolysis products that presented the highest rate constants were V1 and V4 for both compounds. Cytotoxic activity studies using non-tumorigenic HEK293 cell line and human liver cancer HEPG2 cells showed that decavanadates compounds exhibit selectivity action toward HEPG2 cells after 24 h. The e ect of vanadium compounds (8–30 M concentration) on the protein expression of AKT and AMPK were investigated in HEPG2 cell lines, showing that NaDeca and MetfDeca compounds exhibit a dose-dependence increase in phosphorylated AKT. Additionally, NaDeca at 30 M concentration stimulated the glucose cell uptake moderately (62%) in 3T3-L1 adipocytes. Finally, an insulin release assay in TC-6 cells (30 M concentration) showed that sodium orthovanadate (MetV) and MetfDeca enhanced insulin release by 0.7 and 1-fold, respectively.
format Articulo
Articulo
author Silva Nolasco, Aniela M.
Camacho, Luz
Saavedra Díaz, Rafael Omar
Hernández Abreu, Oswaldo
León, Ignacio Esteban
Sánchez Lombardo, Irma
author_facet Silva Nolasco, Aniela M.
Camacho, Luz
Saavedra Díaz, Rafael Omar
Hernández Abreu, Oswaldo
León, Ignacio Esteban
Sánchez Lombardo, Irma
author_sort Silva Nolasco, Aniela M.
title Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity
title_short Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity
title_full Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity
title_fullStr Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity
title_full_unstemmed Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity
title_sort kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin- like activity
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/119728
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