The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis
Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the formation of root nitrogen-fixing nodules. Compatible bacteria can reach cortical cells in a tightly regulated infection process, in which the specific recognition of signal molecules is a key...
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Autores principales: | , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2017
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/119468 |
Aporte de: |
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I19-R120-10915-119468 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Biología Nitrogen fixation Vesicle transport Legume Rhizobia Nodulation |
spellingShingle |
Biología Nitrogen fixation Vesicle transport Legume Rhizobia Nodulation Dalla Via, María Virginia Traubenik, Laura Soledad Rivero, Claudio Aguilar, Orlando Mario Zanetti, María Eugenia Blanco, Flavio Antonio The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis |
topic_facet |
Biología Nitrogen fixation Vesicle transport Legume Rhizobia Nodulation |
description |
Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the formation of root nitrogen-fixing nodules. Compatible bacteria can reach cortical cells in a tightly regulated infection process, in which the specific recognition of signal molecules is a key step to select the symbiotic partner. In this work, we show that RabA2, a monomeric GTPase from common bean, is required for the progression of the infection canal, referred to as the infection thread (IT), toward the cortical cells. Expression of miss-regulated mutant variants of RabA2 resulted in an increased number of abortive infection events, including bursting of ITs and a reduction in the number of nodules. Nodules formed in these plants were small and contained infected cells with disrupted symbiosome membranes, indicating either early senescence of these cells or defects in the formation of the symbiosome membrane during bacterial release. RabA2 localized to mobile vesicles around the IT, but mutations that affect GTP hydrolysis or GTP/GDP exchange modified this localization. Colocalization of RabA2 with ArfA1 and a Golgi marker indicates that RabA2 localizes in Golgi stacks and the trans-Golgi network. Our results suggest that RabA2 is part of the vesicle transport events required to maintain the integrity of the membrane during IT progression. |
format |
Articulo Articulo |
author |
Dalla Via, María Virginia Traubenik, Laura Soledad Rivero, Claudio Aguilar, Orlando Mario Zanetti, María Eugenia Blanco, Flavio Antonio |
author_facet |
Dalla Via, María Virginia Traubenik, Laura Soledad Rivero, Claudio Aguilar, Orlando Mario Zanetti, María Eugenia Blanco, Flavio Antonio |
author_sort |
Dalla Via, María Virginia |
title |
The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis |
title_short |
The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis |
title_full |
The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis |
title_fullStr |
The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis |
title_full_unstemmed |
The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis |
title_sort |
monomeric gtpase raba2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis |
publishDate |
2017 |
url |
http://sedici.unlp.edu.ar/handle/10915/119468 |
work_keys_str_mv |
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Repositorios |
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1764820448446513153 |