DNA methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical DNA

Cancer of the uterine cervix is caused by a subset of oncogenic human Papillomavirus (HPV)-types with mucosal tropism. Besides the known effects of the viral oncoproteins on cellular functions there is evidence suggesting that cervical carcinogenesis involves epigenetic changes in the host DNA. In t...

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Autores principales: García, D. Adriana, Abba, Martín Carlos, Briceño, Ignacio, Aristizabal, Fabio A., Cid Arregui, Angel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Médicas
Cervical cancer
Cervical intraepithelial neoplasia (cin)
Epigenetics
Genome-wide dna-methylation
Gene expression
Human papillomavirus (hpv)
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/119282
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-119282
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Cervical cancer
Cervical intraepithelial neoplasia (cin)
Epigenetics
Genome-wide dna-methylation
Gene expression
Human papillomavirus (hpv)
spellingShingle Ciencias Médicas
Cervical cancer
Cervical intraepithelial neoplasia (cin)
Epigenetics
Genome-wide dna-methylation
Gene expression
Human papillomavirus (hpv)
García, D. Adriana
Abba, Martín Carlos
Briceño, Ignacio
Aristizabal, Fabio A.
Cid Arregui, Angel
DNA methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical DNA
topic_facet Ciencias Médicas
Cervical cancer
Cervical intraepithelial neoplasia (cin)
Epigenetics
Genome-wide dna-methylation
Gene expression
Human papillomavirus (hpv)
description Cancer of the uterine cervix is caused by a subset of oncogenic human Papillomavirus (HPV)-types with mucosal tropism. Besides the known effects of the viral oncoproteins on cellular functions there is evidence suggesting that cervical carcinogenesis involves epigenetic changes in the host DNA. In this study, we have examined the global promoter methylation profile associated with progression to cervical cancer at wide genome scale. The methylation pattern of nearly 14,000 genes was analyzed in cervical swabs at different stages of cervical carcinogenesis: low-grade cervical intraepithelial neoplasia (CIN I and II), high-grade CIN (CIN III) and invasive cancer, as well as healthy individuals. Unsupervised analysis (Hierarchical Clustering) identified two groups: A) healthy, CIN I and CIN II; and B) CIN III/cancer. Supervised T-Test analysis showed 1069 promoter regions hypermethylated and 85 hypomethylated in CIN III/cancer compared to CIN I/CIN II and healthy samples (p<0.0001). Overall, the differentially methylated genes act in transcription, cell cycle, apoptosis and cell adhesion pathways. Of the hypermethylated genes, 132 (12.3%) were down-regulated in a matched cervical cancer group. In turn, only 4 (4.7%) of the hypomethylated genes were overexpressed in that group.
format Articulo
Articulo
author García, D. Adriana
Abba, Martín Carlos
Briceño, Ignacio
Aristizabal, Fabio A.
Cid Arregui, Angel
author_facet García, D. Adriana
Abba, Martín Carlos
Briceño, Ignacio
Aristizabal, Fabio A.
Cid Arregui, Angel
author_sort García, D. Adriana
title DNA methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical DNA
title_short DNA methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical DNA
title_full DNA methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical DNA
title_fullStr DNA methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical DNA
title_full_unstemmed DNA methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical DNA
title_sort dna methylation pattern in high-grade cervical intraepithelial neoplasia and cancer revealed by genomewide methylation analysis of cervical dna
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/119282
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