Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment

The ability to solubilize insoluble inorganic phosphate compounds by Gluconacetobacter diazotrophicus was studied using different culture approaches. Qualitative plate assays using tricalcium phosphate as the sole P-source showed that G. diazotrophicus produced solubilization only when aldoses were...

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Autores principales:	Crespo, Juan Manuel, Boiardi, José Luis, Luna, María Flavia
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Ciencias Agrarias Gluconacetobacter diazotrophicus Phosphate solubilization Glucose dehydrogenase Pqq Biofertilizer
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/119276
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-119276
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Agrarias Gluconacetobacter diazotrophicus Phosphate solubilization Glucose dehydrogenase Pqq Biofertilizer
spellingShingle	Ciencias Agrarias Gluconacetobacter diazotrophicus Phosphate solubilization Glucose dehydrogenase Pqq Biofertilizer Crespo, Juan Manuel Boiardi, José Luis Luna, María Flavia Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment
topic_facet	Ciencias Agrarias Gluconacetobacter diazotrophicus Phosphate solubilization Glucose dehydrogenase Pqq Biofertilizer
description	The ability to solubilize insoluble inorganic phosphate compounds by Gluconacetobacter diazotrophicus was studied using different culture approaches. Qualitative plate assays using tricalcium phosphate as the sole P-source showed that G. diazotrophicus produced solubilization only when aldoses were used as the C-source. Extracellular aldose oxidation via a pyrroloquinoline quinone-linked glucose dehydrogenase (PQQ-GDH) is the main pathway for glucose metabolism in G. diazotrophicus. In batch cultures with 5 g l-1 of hydroxyapatite as the P-source and glucose as the C-source, more than 98% of insoluble P was solubilized. No solubilization was observed neither using glycerol nor culturing a PQQ-GDH mutant of G. diazotrophicus. Solubilizaton was not affected by adding 100 mmol l-1 of MES buffer. Continuous cultures of G. diazotrophicus showed significant activities of PQQ-GDH either under C or P limitation. An intense acidification in the root environment of tomato and wheat seedlings inoculated with a G. diazotrophicus PAL5 was observed. Seedlings inoculated with a PQQ-GDH mutant strain of G. diazotrophicus showed no acidification. Our results suggest that G. diazotrophicus is an excellent candidate to be used as biofertilizer because in addition to the already described plant growth-promoting abilities of this organism, it shows a significant mineral phosphate solubilization capacity.
format	Articulo Articulo
author	Crespo, Juan Manuel Boiardi, José Luis Luna, María Flavia
author_facet	Crespo, Juan Manuel Boiardi, José Luis Luna, María Flavia
author_sort	Crespo, Juan Manuel
title	Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment
title_short	Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment
title_full	Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment
title_fullStr	Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment
title_full_unstemmed	Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment
title_sort	mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under p-limitation and plant root environment
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/119276
work_keys_str_mv	AT crespojuanmanuel mineralphosphatesolubilizationactivityofgluconacetobacterdiazotrophicusunderplimitationandplantrootenvironment AT boiardijoseluis mineralphosphatesolubilizationactivityofgluconacetobacterdiazotrophicusunderplimitationandplantrootenvironment AT lunamariaflavia mineralphosphatesolubilizationactivityofgluconacetobacterdiazotrophicusunderplimitationandplantrootenvironment
bdutipo_str	Repositorios
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Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment

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