CaMKII-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis

Sepsis is associated with cardiac dysfunction, which is at least in part due to cardiomyocyte apoptosis. However, the underlying mechanisms are far from being understood. Using the colon ascendens stent peritonitis mouse model of sepsis (CASP), we examined the subcellular mechanisms that mediate se...

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Detalles Bibliográficos
Autores principales: Sepúlveda, Marisa Noemí, Burgos Migone, Juan Ignacio, Ciocci Pardo, Alejandro, González Arbeláez, Luisa Fernanda, Mosca, Susana María, Vila Petroff, Martín Gerardo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Médicas
Apoptosis
CaMKII
Mitochondrial dysfunction
Ryanodine receptors
Sepsis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/119186
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-119186
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Apoptosis
CaMKII
Mitochondrial dysfunction
Ryanodine receptors
Sepsis
spellingShingle Ciencias Médicas
Apoptosis
CaMKII
Mitochondrial dysfunction
Ryanodine receptors
Sepsis
Sepúlveda, Marisa Noemí
Burgos Migone, Juan Ignacio
Ciocci Pardo, Alejandro
González Arbeláez, Luisa Fernanda
Mosca, Susana María
Vila Petroff, Martín Gerardo
CaMKII-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis
topic_facet Ciencias Médicas
Apoptosis
CaMKII
Mitochondrial dysfunction
Ryanodine receptors
Sepsis
description Sepsis is associated with cardiac dysfunction, which is at least in part due to cardiomyocyte apoptosis. However, the underlying mechanisms are far from being understood. Using the colon ascendens stent peritonitis mouse model of sepsis (CASP), we examined the subcellular mechanisms that mediate sepsis-induced apoptosis. Wildtype (WT) CASP mice hearts showed an increase in apoptosis respect to WT-Sham. CASP transgenic mice expressing a CaMKII inhibitory peptide (AC3-I) were protected against sepsis-induced apoptosis. Dantrolene, used to reduce ryanodine receptor (RyR) diastolic sarcoplasmic reticulum (SR) Ca2+ release, prevented apoptosis in WTCASP. To examine whether CaMKII-dependent RyR2 phosphorylation mediates diastolic Ca2+ release and apoptosis in sepsis, we evaluated apoptosis in mutant mice hearts that have the CaMKII phosphorylation site of RyR2 (Serine 2814) mutated to Alanine (S2814A). S2814A CASP mice did not show increased apoptosis. Consistent with RyR2 phosphorylation-dependent enhancement in diastolic SR Ca2+ release leading to mitochondrial Ca2+ overload, mitochondrial Ca2+ retention capacity was reduced in mitochondria isolated from WT-CASP compared to Sham and this reduction was absent in mitochondria from CASP S2814A or dantrolene-treated mice. We conclude that in sepsis, CaMKII-dependent RyR2 phosphorylation results in diastolic Ca2+ release from SR which leads to mitochondrial Ca2+ overload and apoptosis.
format Articulo
Articulo
author Sepúlveda, Marisa Noemí
Burgos Migone, Juan Ignacio
Ciocci Pardo, Alejandro
González Arbeláez, Luisa Fernanda
Mosca, Susana María
Vila Petroff, Martín Gerardo
author_facet Sepúlveda, Marisa Noemí
Burgos Migone, Juan Ignacio
Ciocci Pardo, Alejandro
González Arbeláez, Luisa Fernanda
Mosca, Susana María
Vila Petroff, Martín Gerardo
author_sort Sepúlveda, Marisa Noemí
title CaMKII-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis
title_short CaMKII-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis
title_full CaMKII-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis
title_fullStr CaMKII-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis
title_full_unstemmed CaMKII-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis
title_sort camkii-dependent ryanodine receptor phosphorylation mediates sepsis-induced cardiomyocyte apoptosis
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/119186
work_keys_str_mv AT sepulvedamarisanoemi camkiidependentryanodinereceptorphosphorylationmediatessepsisinducedcardiomyocyteapoptosis
AT burgosmigonejuanignacio camkiidependentryanodinereceptorphosphorylationmediatessepsisinducedcardiomyocyteapoptosis
AT cioccipardoalejandro camkiidependentryanodinereceptorphosphorylationmediatessepsisinducedcardiomyocyteapoptosis
AT gonzalezarbelaezluisafernanda camkiidependentryanodinereceptorphosphorylationmediatessepsisinducedcardiomyocyteapoptosis
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AT vilapetroffmartingerardo camkiidependentryanodinereceptorphosphorylationmediatessepsisinducedcardiomyocyteapoptosis
bdutipo_str Repositorios
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