[D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues

Two microcystins, MC-LR and [D-Leu1]MC-LR, present in La Plata Basin blooms, are differentiated by substitution of D-Alanine for D-Leucine at position 1. Our objective was to evaluate acute toxicity of [D-Leu1]MC-LR and MC-LR in mice (N:NIH Swiss) and beans (Phaseolus vulgaris). We observed variatio...

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Autores principales:	Sedan, Daniela Yazmine, Malaissi, Luciano, Vaccarini, Cristian Adrián, Ventosi, Ezequiel, Laguens, Martín Ignacio
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2020
Materias:	Ciencias Médicas Química [D-Leu1]Microcystin-LR Microcystin-LR Toxic potency Phosphatase inhibition Phaseolus vulgaris Mice
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/118954
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-118954
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas Química [D-Leu1]Microcystin-LR Microcystin-LR Toxic potency Phosphatase inhibition Phaseolus vulgaris Mice
spellingShingle	Ciencias Médicas Química [D-Leu1]Microcystin-LR Microcystin-LR Toxic potency Phosphatase inhibition Phaseolus vulgaris Mice Sedan, Daniela Yazmine Malaissi, Luciano Vaccarini, Cristian Adrián Ventosi, Ezequiel Laguens, Martín Ignacio [D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues
topic_facet	Ciencias Médicas Química [D-Leu1]Microcystin-LR Microcystin-LR Toxic potency Phosphatase inhibition Phaseolus vulgaris Mice
description	Two microcystins, MC-LR and [D-Leu1]MC-LR, present in La Plata Basin blooms, are differentiated by substitution of D-Alanine for D-Leucine at position 1. Our objective was to evaluate acute toxicity of [D-Leu1]MC-LR and MC-LR in mice (N:NIH Swiss) and beans (Phaseolus vulgaris). We observed variations in [D-Leu1]MC-LR lethal doses with respect to those reported for MC-LR (100 μg/kg), with an increased liver/body weight ratio and intrahepatic hemorrhages in mice exposed to 50–200 μg [D-Leu1]MC-LR/kg and slight steatosis after a single 25 μg [D-Leu1]MC-LR/kg i.p. dose. Our study in the plant model showed alterations in germination, development, morphology and TBARs levels after a single contact with the toxins during imbibition (3.5 and 15 µg/mL), those treated with [D-Leu1]MC-LR being more affected than those treated with the same concentration of MC-LR. Protein phosphatase 1 (PP1) IC50 values were 40.6 nM and 5.3 nM for [D-Leu1]MC-LR and MC-LR, respectively. However, the total phosphatase activity test in root homogenate showed 60% inhibition for [D-Leu1]MC-LR and 12% for MC-LR. In mouse liver homogenate, 50% inhibition was observed for [D-Leu1]MC-LR and 40% for MC-LR. Our findings indicate the need for further research into [D-Leu1]MC-LR toxicity since together with oxidative stress, the possible inhibition of other phosphatases could explain the differences detected in the potency of the two toxins.
format	Articulo Articulo
author	Sedan, Daniela Yazmine Malaissi, Luciano Vaccarini, Cristian Adrián Ventosi, Ezequiel Laguens, Martín Ignacio
author_facet	Sedan, Daniela Yazmine Malaissi, Luciano Vaccarini, Cristian Adrián Ventosi, Ezequiel Laguens, Martín Ignacio
author_sort	Sedan, Daniela Yazmine
title	[D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues
title_short	[D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues
title_full	[D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues
title_fullStr	[D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues
title_full_unstemmed	[D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues
title_sort	[d-leu1]mc-lr has lower pp1 inhibitory capability and greater toxic potency than mc-lr in animal and plant tissues
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/118954
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[D-Leu1]MC-LR Has Lower PP1 Inhibitory Capability and Greater Toxic Potency than MC-LR in Animal and Plant Tissues

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