Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation

The large-scale use of the herbicide glyphosate leads to growing ecotoxicological and human health concerns. Microbe-assisted phytoremediation arises as a good option to remove, contain, or degrade glyphosate from soils and waterbodies, and thus avoid further spreading to non-target areas. To achiev...

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Detalles Bibliográficos
Autores principales:	Massot, Francisco, Gkorezis, Panagiotis, Van Hamme, Jonathan, Marino, Damián José Gabriel, Trifunovic, Bojana Spirovic, Vukovic, Gorica, d’Haen, Jan, Pintelon, Isabel, Giulietti, Ana María, Merini, Luciano, Vangronsveld, Jaco, Thijs, Sofie
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2021
Materias:	Biología Glyphosate Microbe-assisted phytoremediation Epsp synthase Glyphosate tolerance Glyphosate degradation Microcosm Plant-bacteria interaction Phn operon
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/118864
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-118864
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Glyphosate Microbe-assisted phytoremediation Epsp synthase Glyphosate tolerance Glyphosate degradation Microcosm Plant-bacteria interaction Phn operon
spellingShingle	Biología Glyphosate Microbe-assisted phytoremediation Epsp synthase Glyphosate tolerance Glyphosate degradation Microcosm Plant-bacteria interaction Phn operon Massot, Francisco Gkorezis, Panagiotis Van Hamme, Jonathan Marino, Damián José Gabriel Trifunovic, Bojana Spirovic Vukovic, Gorica d’Haen, Jan Pintelon, Isabel Giulietti, Ana María Merini, Luciano Vangronsveld, Jaco Thijs, Sofie Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation
topic_facet	Biología Glyphosate Microbe-assisted phytoremediation Epsp synthase Glyphosate tolerance Glyphosate degradation Microcosm Plant-bacteria interaction Phn operon
description	The large-scale use of the herbicide glyphosate leads to growing ecotoxicological and human health concerns. Microbe-assisted phytoremediation arises as a good option to remove, contain, or degrade glyphosate from soils and waterbodies, and thus avoid further spreading to non-target areas. To achieve this, availability of plant-colonizing, glyphosate-tolerant and -degrading strains is required and at the same time, it must be linked to plant-microorganism interaction studies focusing on a substantive ability to colonize the roots and degrade or transform the herbicide. In this work, we isolated bacteria from a chronically glyphosate-exposed site in Argentina, evaluated their glyphosate tolerance using the minimum inhibitory concentration assay, their in vitro degradation potential, their plant growth-promotion traits, and performed whole genome sequencing to gain insight into the application of a phytoremediation strategy to remediate glyphosate contaminated agronomic soils. Twenty-four soil and root-associated bacterial strains were isolated. Sixteen could grow using glyphosate as the sole source of phosphorous. As shown in MIC assay, some strains tolerated up to 10000 mg kg–1 of glyphosate. Most of them also demonstrated a diverse spectrum of in vitro plant growth-promotion traits, confirmed in their genome sequences. Two representative isolates were studied for their root colonization. An isolate of Ochrobactrum haematophilum exhibited different colonization patterns in the rhizoplane compared to an isolate of Rhizobium sp. Both strains were able to metabolize almost 50% of the original glyphosate concentration of 50 mg l–1 in 9 days. In a microcosms experiment with Lotus corniculatus L, O. haematophilum performed better than Rhizobium, with 97% of glyphosate transformed after 20 days. The results suggest that L. corniculatus in combination with to O. haematophilum can be adopted for phytoremediation of glyphosate on agricultural soils. An effective strategy is presented of linking the experimental data from the isolation of tolerant bacteria with performing plant-bacteria interaction tests to demonstrate positive effects on the removal of glyphosate from soils.
format	Articulo Articulo
author	Massot, Francisco Gkorezis, Panagiotis Van Hamme, Jonathan Marino, Damián José Gabriel Trifunovic, Bojana Spirovic Vukovic, Gorica d’Haen, Jan Pintelon, Isabel Giulietti, Ana María Merini, Luciano Vangronsveld, Jaco Thijs, Sofie
author_facet	Massot, Francisco Gkorezis, Panagiotis Van Hamme, Jonathan Marino, Damián José Gabriel Trifunovic, Bojana Spirovic Vukovic, Gorica d’Haen, Jan Pintelon, Isabel Giulietti, Ana María Merini, Luciano Vangronsveld, Jaco Thijs, Sofie
author_sort	Massot, Francisco
title	Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation
title_short	Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation
title_full	Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation
title_fullStr	Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation
title_full_unstemmed	Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation
title_sort	isolation, biochemical and genomic characterization of glyphosate tolerant bacteria to perform microbe-assisted phytoremediation
publishDate	2021
url	http://sedici.unlp.edu.ar/handle/10915/118864
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bdutipo_str	Repositorios
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Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation

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