Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis

Nuclear-lipid droplets (nLD)—a dynamic cellular organelle that stores neutral lipids, within the nucleus of eukaryotic cells—consists of a hydrophobic triacylglycerol –cholesterol-ester core enriched in oleic acid (OA) surrounded by a monolayer of polar lipids, cholesterol, and proteins. nLD are pro...

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Detalles Bibliográficos
Autores principales: Lagrutta, Lucía Carolina, Layerenza, Juan Pablo, Bronsoms, Silvia, Trejo, Sebastián Alejandro, Ves Losada, Ana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Biología
Nuclear-lipid droplets
Proteomic
Nuclear proteins
Lipid metabolism
Lipase
Carboxylesterase
Triacylglyceride
Cholesterol-ester
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118818
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118818
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Nuclear-lipid droplets
Proteomic
Nuclear proteins
Lipid metabolism
Lipase
Carboxylesterase
Triacylglyceride
Cholesterol-ester
spellingShingle Biología
Nuclear-lipid droplets
Proteomic
Nuclear proteins
Lipid metabolism
Lipase
Carboxylesterase
Triacylglyceride
Cholesterol-ester
Lagrutta, Lucía Carolina
Layerenza, Juan Pablo
Bronsoms, Silvia
Trejo, Sebastián Alejandro
Ves Losada, Ana
Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis
topic_facet Biología
Nuclear-lipid droplets
Proteomic
Nuclear proteins
Lipid metabolism
Lipase
Carboxylesterase
Triacylglyceride
Cholesterol-ester
description Nuclear-lipid droplets (nLD)—a dynamic cellular organelle that stores neutral lipids, within the nucleus of eukaryotic cells—consists of a hydrophobic triacylglycerol –cholesterol-ester core enriched in oleic acid (OA) surrounded by a monolayer of polar lipids, cholesterol, and proteins. nLD are probably involved in nuclear-lipid homeostasis serving as an endonuclear buffer that provides or incorporates lipids and proteins participating in signaling pathways, as transcription factors and enzymes of lipid metabolism and nuclear processes. In the present work, we analyzed the nLD proteome and hypothesized that nLD-monolayer proteins could be involved in processes similar as the ones occurring in the cLD including lipid metabolism and other cellular functions. We evaluated the rat-liver–nLD proteome under physiological and nonpathological conditions by GeLC-MS2. Since isolated nLD are highly diluted, a protein-concentrating isolation protocol was designed. Thirty-five proteins were identified within the functional categories: cytoskeleton and structural, transcription and translation, histones, protein-folding and posttranslational modification, cellular proliferation and/or cancer, lipid metabolism, and transport. Purified nLD contained an enzyme from the lipid-metabolism pathway, carboxylesterase 1d (Ces1d/ Ces3). Nuclear Carboxylesterase localization was confirmed by Western blotting. By in-silico analyses rat Ces1d/ Ces3 secondary and tertiary structure predicted would be equivalent to human CES1. These results—the first nLD proteome—demonstrate that a tandem-GeLC-MS2-analysis protocol facilitates studies like these on rat-liver nuclei. A diversity of cellular-protein function was identified indicating the direct or indirect nLD participation and involving Ces1d/Ces3 in the LD-population homeostasis.
format Articulo
Articulo
author Lagrutta, Lucía Carolina
Layerenza, Juan Pablo
Bronsoms, Silvia
Trejo, Sebastián Alejandro
Ves Losada, Ana
author_facet Lagrutta, Lucía Carolina
Layerenza, Juan Pablo
Bronsoms, Silvia
Trejo, Sebastián Alejandro
Ves Losada, Ana
author_sort Lagrutta, Lucía Carolina
title Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis
title_short Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis
title_full Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis
title_fullStr Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis
title_full_unstemmed Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis
title_sort nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/118818
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