A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects

Background & aims: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disorder, tightly associated with obesity. The histological spectrum of the disease ranges from simple steatosis to steatohepatitis, with different stages of fibrosis, and fibrosis stage is the most significant p...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Giraudi, Pablo, Gambaro, Sabrina Eliana, Ornelas Arroyo, Sofia, Chackelevicius, Carla M., Giuricin, Michela, Silvestri, Marta, Macor, Daniele, Crocé, Lory S., Bonazza, Deborah, Soardo, Giorgio, Manzini, Nicolò de, Zanconati, Fabrizio, Tiribelli, Claudio, Palmisano, Silvia, Rosso, Natalia
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Biomarkers
In silico strategy
Liver fibrosis
Morbidly obese
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118761
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118761
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biomarkers
In silico strategy
Liver fibrosis
Morbidly obese
spellingShingle Biología
Biomarkers
In silico strategy
Liver fibrosis
Morbidly obese
Giraudi, Pablo
Gambaro, Sabrina Eliana
Ornelas Arroyo, Sofia
Chackelevicius, Carla M.
Giuricin, Michela
Silvestri, Marta
Macor, Daniele
Crocé, Lory S.
Bonazza, Deborah
Soardo, Giorgio
Manzini, Nicolò de
Zanconati, Fabrizio
Tiribelli, Claudio
Palmisano, Silvia
Rosso, Natalia
A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
topic_facet Biología
Biomarkers
In silico strategy
Liver fibrosis
Morbidly obese
description Background & aims: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disorder, tightly associated with obesity. The histological spectrum of the disease ranges from simple steatosis to steatohepatitis, with different stages of fibrosis, and fibrosis stage is the most significant predictor of mortality in NAFLD. Liver biopsy continues to be the gold standard for its diagnosis and reliable non-invasive diagnostic tools are unavailable. We investigated the accuracy of candidate proteins, identified by an in silico approach, as biomarkers for diagnosis of fibrosis. Methods: Seventy-one morbidly obese (MO) subjects with biopsy-proven NAFLD were enrolled, and the cohort was subdivided according to minimal (F0/F1) or moderate (F2/F3) fibrosis. The plasmatic level of CD44 antigen (CD44), secreted protein acidic and rich in cysteine (SPARC), epidermal growth factor receptor (EGFR) and insulin-like growth factor 2 (IGF2) were determined by ELISA. Significant associations between plasmatic levels and histological fibrosis were determined by correlation analysis and the diagnostic accuracy by the area under receiver operating characteristic curves (AUROC). Results: Eighty-two percentage of the subjects had F0/F1 and 18% with F2/F3 fibrosis. Plasmatic levels of IGF2, EGFR and their ratio (EGFR/IGF2) were associated with liver fibrosis, correlating inversely for IGF2 (P < .006) and directly (P < .018; P < .0001) for EGFR and EGFR/IGF2 respectively. The IGF2 marker had the best diagnostic accuracy for moderate fibrosis (AUROC 0.83), followed by EGFR/IGF2 ratio (AUROC 0.79) and EGFR (AUROC 0.71). Conclusions: Our study supports the potential utility of IGF2 and EGFR as non-invasive diagnostic biomarkers for liver fibrosis in morbidly obese subjects.
format Articulo
Preprint
author Giraudi, Pablo
Gambaro, Sabrina Eliana
Ornelas Arroyo, Sofia
Chackelevicius, Carla M.
Giuricin, Michela
Silvestri, Marta
Macor, Daniele
Crocé, Lory S.
Bonazza, Deborah
Soardo, Giorgio
Manzini, Nicolò de
Zanconati, Fabrizio
Tiribelli, Claudio
Palmisano, Silvia
Rosso, Natalia
author_facet Giraudi, Pablo
Gambaro, Sabrina Eliana
Ornelas Arroyo, Sofia
Chackelevicius, Carla M.
Giuricin, Michela
Silvestri, Marta
Macor, Daniele
Crocé, Lory S.
Bonazza, Deborah
Soardo, Giorgio
Manzini, Nicolò de
Zanconati, Fabrizio
Tiribelli, Claudio
Palmisano, Silvia
Rosso, Natalia
author_sort Giraudi, Pablo
title A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
title_short A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
title_full A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
title_fullStr A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
title_full_unstemmed A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
title_sort simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/118761
work_keys_str_mv AT giraudipablo asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT gambarosabrinaeliana asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT ornelasarroyosofia asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT chackeleviciuscarlam asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT giuricinmichela asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT silvestrimarta asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT macordaniele asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT crocelorys asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT bonazzadeborah asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT soardogiorgio asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT manzininicolode asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT zanconatifabrizio asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT tiribelliclaudio asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT palmisanosilvia asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT rossonatalia asimpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT giraudipablo simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT gambarosabrinaeliana simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT ornelasarroyosofia simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT chackeleviciuscarlam simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT giuricinmichela simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT silvestrimarta simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT macordaniele simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT crocelorys simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT bonazzadeborah simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT soardogiorgio simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT manzininicolode simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT zanconatifabrizio simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT tiribelliclaudio simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT palmisanosilvia simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
AT rossonatalia simpleinsilicostrategyidentifiescandidatebiomarkersforthediagnosisofliverfibrosisinmorbidlyobesesubjects
bdutipo_str Repositorios
_version_ 1764820447536349185

Ejemplares similares