Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury

Scar formation after brain injury is still poorly understood. To further elucidate such processes, here, we examine the interplay between astrocyte proliferation taking place predominantly at the vascular interface and monocyte invasion. Using genetic mouse models that decrease or increase reactive...

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Autores principales:	Frik, Jesica, Merl-Pham, Juliane, Plesnila, Nikolaus, Mattugini, Nicola, Kjell, Jacob, Kraska, Jonas, Gómez, Ricardo Martín, Hauck, Stefanie M., Sirko, Swetlana, Götz, Magdalena
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2018
Materias:	Biología aryl hydrocarbon receptor astrogliosis monocytes scar formation sonic hedgehog pathway traumatic brain injury
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/118630
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-118630
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología aryl hydrocarbon receptor astrogliosis monocytes scar formation sonic hedgehog pathway traumatic brain injury
spellingShingle	Biología aryl hydrocarbon receptor astrogliosis monocytes scar formation sonic hedgehog pathway traumatic brain injury Frik, Jesica Merl-Pham, Juliane Plesnila, Nikolaus Mattugini, Nicola Kjell, Jacob Kraska, Jonas Gómez, Ricardo Martín Hauck, Stefanie M. Sirko, Swetlana Götz, Magdalena Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
topic_facet	Biología aryl hydrocarbon receptor astrogliosis monocytes scar formation sonic hedgehog pathway traumatic brain injury
description	Scar formation after brain injury is still poorly understood. To further elucidate such processes, here, we examine the interplay between astrocyte proliferation taking place predominantly at the vascular interface and monocyte invasion. Using genetic mouse models that decrease or increase reactive astrocyte proliferation, we demonstrate inverse effects on monocyte numbers in the injury site. Conversely, reducing monocyte invasion using CCR2-/- mice causes a strong increase in astrocyte proliferation, demonstrating an intriguing negative cross-regulation between these cell types at the vascular interface. CCR2-/- mice show reduced scar formation with less extracellular matrix deposition, smaller lesion site and increased neuronal coverage. Surprisingly, the GFAP+ scar area in these mice is also significantly decreased despite increased astrocyte proliferation. Proteomic analysis at the peak of increased astrocyte proliferation reveals a decrease in extracellular matrix synthesizing enzymes in the injury sites of CCR2-/- mice, highlighting how early key aspects of scar formation are initiated. Taken together, we provide novel insights into the cross-regulation of juxtavascular proliferating astrocytes and invading monocytes as a crucial mechanism of scar formation upon brain injury.
format	Articulo Articulo
author	Frik, Jesica Merl-Pham, Juliane Plesnila, Nikolaus Mattugini, Nicola Kjell, Jacob Kraska, Jonas Gómez, Ricardo Martín Hauck, Stefanie M. Sirko, Swetlana Götz, Magdalena
author_facet	Frik, Jesica Merl-Pham, Juliane Plesnila, Nikolaus Mattugini, Nicola Kjell, Jacob Kraska, Jonas Gómez, Ricardo Martín Hauck, Stefanie M. Sirko, Swetlana Götz, Magdalena
author_sort	Frik, Jesica
title	Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
title_short	Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
title_full	Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
title_fullStr	Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
title_full_unstemmed	Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
title_sort	cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
publishDate	2018
url	http://sedici.unlp.edu.ar/handle/10915/118630
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bdutipo_str	Repositorios
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Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury

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