Lipid peroxidation assays in canine fresh semen

Lipid peroxidation (LP) is a potential cause of infertility in males of numerous species. Levels in which the spermatozoa loses mobility in vitro is correlated with the rate of LP that it suffers. The objective of this work was to determine the fatty acid composition and analyze the sensitivity to L...

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Autores principales: Gavazza, Mariana Beatriz, Gutiérrez, Ana María, Marmunti, Monica Edith, Palacios, Alejandro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Veterinarias
Dog
Spermatozoa
Lipid peroxidation
Polyunsaturated fatty acids
Perros
Espermatozoides
Peroxidación lipídica
Ácidos grasos poliinsaturados
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/116110
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-116110
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Dog
Spermatozoa
Lipid peroxidation
Polyunsaturated fatty acids
Perros
Espermatozoides
Peroxidación lipídica
Ácidos grasos poliinsaturados
spellingShingle Ciencias Veterinarias
Dog
Spermatozoa
Lipid peroxidation
Polyunsaturated fatty acids
Perros
Espermatozoides
Peroxidación lipídica
Ácidos grasos poliinsaturados
Gavazza, Mariana Beatriz
Gutiérrez, Ana María
Marmunti, Monica Edith
Palacios, Alejandro
Lipid peroxidation assays in canine fresh semen
topic_facet Ciencias Veterinarias
Dog
Spermatozoa
Lipid peroxidation
Polyunsaturated fatty acids
Perros
Espermatozoides
Peroxidación lipídica
Ácidos grasos poliinsaturados
description Lipid peroxidation (LP) is a potential cause of infertility in males of numerous species. Levels in which the spermatozoa loses mobility in vitro is correlated with the rate of LP that it suffers. The objective of this work was to determine the fatty acid composition and analyze the sensitivity to LP (ascorbate–Fe++ dependent) in spermatozoa obtained from different samples of canine fresh semen. LP was evaluated using chemiluminescence (CL) (cpm/mg of protein) and fatty acid (FA) profile by means of gas chromatography. The saturated FA content found in the analyzed spermatozoa was approximately 40%, whereas the total unsaturated FA content was approximately 55% with a prevalence of docosapentaenoic acid (C22:5 n6). When the control and ascorbate–Fe++ dependent samples were compared, it was observed a significant increase in the light emission (CL). Consequently, significant decrease in the percentage of the polyunsaturated fatty acids (PUFA) was obtained, being more affected: C18: 2 n6, C20: 4 n6, C22: 4 n6, C22: 5 n6 and C22: 6 n3. The unsaturation index used to evaluate the alterations generated during the LP was correlated with the data mentioned before. Our results indicate that dog semen contains great amounts of PUFA, which were vulnerable to the LP. The alteration in the PUFA composition could be the common base of different degenerative processes.
format Articulo
Articulo
author Gavazza, Mariana Beatriz
Gutiérrez, Ana María
Marmunti, Monica Edith
Palacios, Alejandro
author_facet Gavazza, Mariana Beatriz
Gutiérrez, Ana María
Marmunti, Monica Edith
Palacios, Alejandro
author_sort Gavazza, Mariana Beatriz
title Lipid peroxidation assays in canine fresh semen
title_short Lipid peroxidation assays in canine fresh semen
title_full Lipid peroxidation assays in canine fresh semen
title_fullStr Lipid peroxidation assays in canine fresh semen
title_full_unstemmed Lipid peroxidation assays in canine fresh semen
title_sort lipid peroxidation assays in canine fresh semen
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/116110
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bdutipo_str Repositorios
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