Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes

Background: Reactive oxygen species, such as superoxide, are being increasingly recognized as key components of a vast array of signaling pathways. Angiotensin II is a well-recognized stimulus for superoxide production through NADPH oxidase activation and opening of the mitochondrial ATP-sensitive p...

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Autores principales:	Yeves, Alejandra del Milagro, Caldiz, Claudia Irma, Aiello, Ernesto Alejandro, Villa Abrille, María Celeste, Ennis, Irene Lucía
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2015
Materias:	Medicina Angiotensin II Inotropism Reactive oxygen species NHE-1 Isolated cardiomyocytes
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/114769
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-114769
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Medicina Angiotensin II Inotropism Reactive oxygen species NHE-1 Isolated cardiomyocytes
spellingShingle	Medicina Angiotensin II Inotropism Reactive oxygen species NHE-1 Isolated cardiomyocytes Yeves, Alejandra del Milagro Caldiz, Claudia Irma Aiello, Ernesto Alejandro Villa Abrille, María Celeste Ennis, Irene Lucía Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes
topic_facet	Medicina Angiotensin II Inotropism Reactive oxygen species NHE-1 Isolated cardiomyocytes
description	Background: Reactive oxygen species, such as superoxide, are being increasingly recognized as key components of a vast array of signaling pathways. Angiotensin II is a well-recognized stimulus for superoxide production through NADPH oxidase activation and opening of the mitochondrial ATP-sensitive potassium channels (mKATP). A role for this mechanism has been proposed to explain several physiological effects of the peptide. The aim of this study was to evaluate the involvement of this mechanism in the inotropic response to 100 nmol/L angiotensin II. Methods: Sarcomere shortening and intracellular pH (BCECF-epifluorescence technique) were evaluated in isolated cat ventricular myocytes placed in a perfusion chamber on the stage of an inverted microscope. Myocardial superoxide production was evaluated by the lucigenin quimioluminiscence method. Results: Angiotensin II (100 nmol/L) increased~70% sarcomere shortening, effect that was only partially prevented by NADPH oxidase inhibition,mKATP channel blockade or inhibition of the cardiac Na+/H+ exchanger (NHE-1). Moreover, angiotensin II stimulates NHE-1 activity by a NADPH oxidase-dependent mechanism. Myocardial superoxide production was also increased by angiotensin II, and this action was completely prevented either by NADPH oxidase inhibition or mKATP channel blockade. Conclusions: The positive inotropic response to 100 nmol/L angiotensin II is due to both ROS/NHE-1 dependent and independent pathways, this being a point of divergence with the signaling previously described to be triggered by lower concentrations of angiotensin II (i.e.: 1 nmol/L).
format	Articulo Articulo
author	Yeves, Alejandra del Milagro Caldiz, Claudia Irma Aiello, Ernesto Alejandro Villa Abrille, María Celeste Ennis, Irene Lucía
author_facet	Yeves, Alejandra del Milagro Caldiz, Claudia Irma Aiello, Ernesto Alejandro Villa Abrille, María Celeste Ennis, Irene Lucía
author_sort	Yeves, Alejandra del Milagro
title	Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes
title_short	Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes
title_full	Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes
title_fullStr	Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes
title_full_unstemmed	Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes
title_sort	reactive oxygen species partially mediate high dose angiotensin ii-induced positive inotropic effect in cat ventricular myocytes
publishDate	2015
url	http://sedici.unlp.edu.ar/handle/10915/114769
work_keys_str_mv	AT yevesalejandradelmilagro reactiveoxygenspeciespartiallymediatehighdoseangiotensiniiinducedpositiveinotropiceffectincatventricularmyocytes AT caldizclaudiairma reactiveoxygenspeciespartiallymediatehighdoseangiotensiniiinducedpositiveinotropiceffectincatventricularmyocytes AT aielloernestoalejandro reactiveoxygenspeciespartiallymediatehighdoseangiotensiniiinducedpositiveinotropiceffectincatventricularmyocytes AT villaabrillemariaceleste reactiveoxygenspeciespartiallymediatehighdoseangiotensiniiinducedpositiveinotropiceffectincatventricularmyocytes AT ennisirenelucia reactiveoxygenspeciespartiallymediatehighdoseangiotensiniiinducedpositiveinotropiceffectincatventricularmyocytes
bdutipo_str	Repositorios
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Reactive oxygen species partially mediate high dose angiotensin II-induced positive inotropic effect in cat ventricular myocytes

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