Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays

Canine monocytic ehrlichiosis (CME) is an infectious disease caused by the bacterium Ehrlichia canis and transmitted by Rhipicephalus sanguineus sensu lato, a tick with worldwide distribution. When not diagnosed and treated early, disease can be severe. Currently, the disease is confirmed by serolo...

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Autores principales: Martín, Paula Lorena, De Salvo, María N., Cicuttin, Gabriel L., Arauz, María Sandra
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Veterinarias
Canine
Monocytic ehrlichiosis
Buenos Aires
Argentina
Serology
Molecular assay
Dogs
Diagnosis
Ehrlichiosis
PCR,
Bacterioses
Ehrlichiose monocítica
Teste serológico
Teste molecular
Cães
Diagnóstico
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/109895
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-109895
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Canine
Monocytic ehrlichiosis
Buenos Aires
Argentina
Serology
Molecular assay
Dogs
Diagnosis
Ehrlichiosis
PCR,
Bacterioses
Ehrlichiose monocítica
Teste serológico
Teste molecular
Cães
Diagnóstico
spellingShingle Ciencias Veterinarias
Canine
Monocytic ehrlichiosis
Buenos Aires
Argentina
Serology
Molecular assay
Dogs
Diagnosis
Ehrlichiosis
PCR,
Bacterioses
Ehrlichiose monocítica
Teste serológico
Teste molecular
Cães
Diagnóstico
Martín, Paula Lorena
De Salvo, María N.
Cicuttin, Gabriel L.
Arauz, María Sandra
Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays
topic_facet Ciencias Veterinarias
Canine
Monocytic ehrlichiosis
Buenos Aires
Argentina
Serology
Molecular assay
Dogs
Diagnosis
Ehrlichiosis
PCR,
Bacterioses
Ehrlichiose monocítica
Teste serológico
Teste molecular
Cães
Diagnóstico
description Canine monocytic ehrlichiosis (CME) is an infectious disease caused by the bacterium Ehrlichia canis and transmitted by Rhipicephalus sanguineus sensu lato, a tick with worldwide distribution. When not diagnosed and treated early, disease can be severe. Currently, the disease is confirmed by serological or molecular assays. The objective of this study was to compare a serological assay based on immunochromatography (SPEED® EHRLI immunochromatographic test; BVT, France) and a molecular assay (a screening PCR followed by a nested PCR specific for E. canis) for the diagnosis of E. canis in suspected dogs from Buenos Aires city and southern Greater Buenos Aires, Argentina. Blood samples from 20 clinically healthy dogs (Control Group) and from 80 sick dogs suspected of having CME (Groups 1 to 4) were tested in parallel. Neither the immunochromatographic test nor the PCR assay was able to detect the presence of E. canis in the Control Group. In the group which had been previously tested by serology, the agreement between the tests was low (kappa: 0.200), whereas in the group which had been previously tested by PCR, the concordance between the tests was adequate (kappa: 0.650). The concordance between the tests evaluated in the total population studied was moderate (kappa: 0.496). The results of our study suggest that the use of rapid serological tests as a first approach, together with subsequent confirmation by PCR, will improve the diagnosis of CME.
format Articulo
Articulo
author Martín, Paula Lorena
De Salvo, María N.
Cicuttin, Gabriel L.
Arauz, María Sandra
author_facet Martín, Paula Lorena
De Salvo, María N.
Cicuttin, Gabriel L.
Arauz, María Sandra
author_sort Martín, Paula Lorena
title Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays
title_short Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays
title_full Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays
title_fullStr Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays
title_full_unstemmed Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays
title_sort canine monocytic ehrlichiosis in buenos aires, argentina: comparison of serological and molecular assays
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/109895
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