Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis
Citrus tristeza closterovirus (CTV), is a phloem-limited virus transmitted by aphids in a semipersistent manner. The genome of CTV is composed of a ssRNA with two capsid proteins: CP, covering about 95% of the particle length, and a diverged coat protein (dCP), present only in one end of the particl...
Guardado en:
| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1999
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/108613 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v2n1-3 |
| Aporte de: |
| id |
I19-R120-10915-108613 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Citrus Tristeza Virus Single-strand conformation polymorphism Genome |
| spellingShingle |
Bioquímica Citrus Tristeza Virus Single-strand conformation polymorphism Genome Gago Zachert, Selma Pérsida Costa, Norma B. Semorile, Liliana Carmen Grau, Oscar Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis |
| topic_facet |
Bioquímica Citrus Tristeza Virus Single-strand conformation polymorphism Genome |
| description |
Citrus tristeza closterovirus (CTV), is a phloem-limited virus transmitted by aphids in a semipersistent manner. The genome of CTV is composed of a ssRNA with two capsid proteins: CP, covering about 95% of the particle length, and a diverged coat protein (dCP), present only in one end of the particle, forming a rattlesnake structure. dCP is the product of p27 gene for which it is also postulated a function in the transmissibility by aphid vectors. Hybridization analysis showed a p27 gene region, which exhibits different patterns with two probes derived from two biological distinct CTV isolates. In an attempt to screen whether that gene region differs in mild and severe strains, six CTV isolates belonging to different biogroups were compared for variations in their p27 gene by analysis of single-strand conformation polymorphism (SSCP). The p27 gene was reverse transcribed and amplified by PCR and thirty clones of each isolate were obtained. From each clone, two fragments of the gene were amplified by PCR: fragment (a), 459 bp long, and fragment (b), 281 bp long. Sequence variations in both gene fragments were studied by SSCP analysis. A variety of SSCP patterns was obtained from each isolate, being isolates belonging to the groups II-IV and III those with the higher and lower number of them. Moreover, SSCP analysis provided a rapid procedure to screen the genetic heterogeneity of the viral isolates reducing considerably the amount of nucleic acid sequenciation necessary to gain that knowledge. |
| format |
Articulo Articulo |
| author |
Gago Zachert, Selma Pérsida Costa, Norma B. Semorile, Liliana Carmen Grau, Oscar |
| author_facet |
Gago Zachert, Selma Pérsida Costa, Norma B. Semorile, Liliana Carmen Grau, Oscar |
| author_sort |
Gago Zachert, Selma Pérsida |
| title |
Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis |
| title_short |
Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis |
| title_full |
Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis |
| title_fullStr |
Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis |
| title_full_unstemmed |
Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis |
| title_sort |
sequence variability in p27 gene of citrus tristeza virus (ctv) revealed by sscp analysis |
| publishDate |
1999 |
| url |
http://sedici.unlp.edu.ar/handle/10915/108613 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v2n1-3 |
| work_keys_str_mv |
AT gagozachertselmapersida sequencevariabilityinp27geneofcitrustristezavirusctvrevealedbysscpanalysis AT costanormab sequencevariabilityinp27geneofcitrustristezavirusctvrevealedbysscpanalysis AT semorilelilianacarmen sequencevariabilityinp27geneofcitrustristezavirusctvrevealedbysscpanalysis AT grauoscar sequencevariabilityinp27geneofcitrustristezavirusctvrevealedbysscpanalysis |
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Repositorios |
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1764820443851653121 |