<i>Citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes

Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: De Francesco, Agustina, Costa, N., García, María Laura
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Citrus psorosis virus
Hairpin-RNA
Psorosis A
Psorosis B
Transgenic sweet orange
Virus resistance
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/108516
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-108516
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Citrus psorosis virus
Hairpin-RNA
Psorosis A
Psorosis B
Transgenic sweet orange
Virus resistance
spellingShingle Ciencias Exactas
Citrus psorosis virus
Hairpin-RNA
Psorosis A
Psorosis B
Transgenic sweet orange
Virus resistance
De Francesco, Agustina
Costa, N.
García, María Laura
<i>Citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
topic_facet Ciencias Exactas
Citrus psorosis virus
Hairpin-RNA
Psorosis A
Psorosis B
Transgenic sweet orange
Virus resistance
description Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP- 15 manifested complete resistance while line ihpCP- 10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis.
format Articulo
Articulo
author De Francesco, Agustina
Costa, N.
García, María Laura
author_facet De Francesco, Agustina
Costa, N.
García, María Laura
author_sort De Francesco, Agustina
title <i>Citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
title_short <i>Citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
title_full <i>Citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
title_fullStr <i>Citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
title_full_unstemmed <i>Citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
title_sort <i>citrus psorosis virus</i> coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis a and b syndromes
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/108516
work_keys_str_mv AT defrancescoagustina icitruspsorosisvirusicoatproteinderivedhairpinconstructconfersstabletransgenicresistanceincitrusagainstpsorosisaandbsyndromes
AT costan icitruspsorosisvirusicoatproteinderivedhairpinconstructconfersstabletransgenicresistanceincitrusagainstpsorosisaandbsyndromes
AT garciamarialaura icitruspsorosisvirusicoatproteinderivedhairpinconstructconfersstabletransgenicresistanceincitrusagainstpsorosisaandbsyndromes
bdutipo_str Repositorios
_version_ 1764820443766718468

Ejemplares similares