Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion

Amaranth protein isolate was obtained and subjected to simulated gastrointestinal digestion to evaluate its potential antithrombotic activity. The protein isolate did not present fibrin clotting inhibition at the concentrations studied, whereas the hydrolysate (DH% = 51.1 ± 3.8%) exhibited inhibitio...

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Detalles Bibliográficos
Autores principales:	Sabbione, Ana Clara, Nardo, Agustina Estefanía, Añón, María Cristina, Scilingo, Adriana Alicia
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2015
Materias:	Ciencias Exactas Amaranth protein isolate Gastrointestinal digestion Antithrombotic activity Absorption by Caco 2 cell culture Bioactive peptides
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/108335
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-108335
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Amaranth protein isolate Gastrointestinal digestion Antithrombotic activity Absorption by Caco 2 cell culture Bioactive peptides
spellingShingle	Ciencias Exactas Amaranth protein isolate Gastrointestinal digestion Antithrombotic activity Absorption by Caco 2 cell culture Bioactive peptides Sabbione, Ana Clara Nardo, Agustina Estefanía Añón, María Cristina Scilingo, Adriana Alicia Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion
topic_facet	Ciencias Exactas Amaranth protein isolate Gastrointestinal digestion Antithrombotic activity Absorption by Caco 2 cell culture Bioactive peptides
description	Amaranth protein isolate was obtained and subjected to simulated gastrointestinal digestion to evaluate its potential antithrombotic activity. The protein isolate did not present fibrin clotting inhibition at the concentrations studied, whereas the hydrolysate (DH% = 51.1 ± 3.8%) exhibited inhibition of fibrin coagulation, showing a dose–response behaviour (IC<i>50</i> = 0.23 ± 0.02 mg/mL), confirming that the enzymatic treatment was able to release bioactive peptides from amaranth proteins. A fraction with high antithrombotic activity was obtained from this hydrolysate, and resulted to be three times more potent than its original sample (IC<i>50</i> = 0.07 ± 0.01 mg/mL). The absorption of this active fraction was studied with an in vitro peptides transport assay through intestinal epithelium and it was observed that some peptides are able to cross the Caco2-TC7 cell monolayer. Potentially bioactive peptides were found after sequencing them, and informatics tools allowed us to select and locate in their native molecules those peptides prone to inhibit thrombin activity.
format	Articulo Articulo
author	Sabbione, Ana Clara Nardo, Agustina Estefanía Añón, María Cristina Scilingo, Adriana Alicia
author_facet	Sabbione, Ana Clara Nardo, Agustina Estefanía Añón, María Cristina Scilingo, Adriana Alicia
author_sort	Sabbione, Ana Clara
title	Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion
title_short	Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion
title_full	Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion
title_fullStr	Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion
title_full_unstemmed	Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion
title_sort	amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion
publishDate	2015
url	http://sedici.unlp.edu.ar/handle/10915/108335
work_keys_str_mv	AT sabbioneanaclara amaranthpeptideswithantithromboticactivityreleasedbysimulatedgastrointestinaldigestion AT nardoagustinaestefania amaranthpeptideswithantithromboticactivityreleasedbysimulatedgastrointestinaldigestion AT anonmariacristina amaranthpeptideswithantithromboticactivityreleasedbysimulatedgastrointestinaldigestion AT scilingoadrianaalicia amaranthpeptideswithantithromboticactivityreleasedbysimulatedgastrointestinaldigestion
bdutipo_str	Repositorios
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Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion

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