Protein composition of the occlusion bodies of Epinotia aporema granulovirus

Within family Baculoviridae, members of the Betabaculovirus genus are employed as biocontrol agents against lepidopteran pests, either alone or in combination with selected members of the Alphabaculovirus genus. Epinotia aporema granulovirus (...

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Detalles Bibliográficos
Autores principales:	Masson, Tomás, Fabre, María Laura, Ferrelli, María Leticia, Pidre, Matías Luis, Romanowski, Víctor
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2019
Materias:	Ciencias Exactas Biología proteomes virions Genomics proteomic databases gene prediction baculoviruses larvae invertebrate genomics
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107807 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6372164&blobtype=pdf https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207735
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107807
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Biología proteomes virions Genomics proteomic databases gene prediction baculoviruses larvae invertebrate genomics
spellingShingle	Ciencias Exactas Biología proteomes virions Genomics proteomic databases gene prediction baculoviruses larvae invertebrate genomics Masson, Tomás Fabre, María Laura Ferrelli, María Leticia Pidre, Matías Luis Romanowski, Víctor Protein composition of the occlusion bodies of <i>Epinotia aporema</i> granulovirus
topic_facet	Ciencias Exactas Biología proteomes virions Genomics proteomic databases gene prediction baculoviruses larvae invertebrate genomics
description	Within family Baculoviridae, members of the <i>Betabaculovirus</i> genus are employed as biocontrol agents against lepidopteran pests, either alone or in combination with selected members of the <i>Alphabaculovirus</i> genus. <i>Epinotia aporema</i> granulovirus (EpapGV) is a fast killing betabaculovirus that infects the bean shoot borer (<i>E. aporema</i>) and is a promising biopesticide. Because occlusion bodies (OBs) play a key role in baculovirus horizontal transmission, we investigated the composition of EpapGV OBs. Using mass spectrometry-based proteomics we could identify 56 proteins that are included in the OBs during the final stages of larval infection. Our data provides experimental validation of several annotated hypothetical coding sequences. Proteogenomic mapping against genomic sequence detected a previously unannotated ac<i>110</i>-like core gene and a putative translation fusion product of ORFs <i>epap48</i> and <i>epap49</i>. Comparative studies of the proteomes available for the family <i>Baculoviridae</i> highlight the conservation of core gene products as parts of the occluded virion. Two proteins specific for betabaculoviruses (Epap48 and Epap95) are incorporated into OBs. Moreover, quantification based on emPAI values showed that Epap95 is one of the most abundant components of EpapGV OBs.
format	Articulo Articulo
author	Masson, Tomás Fabre, María Laura Ferrelli, María Leticia Pidre, Matías Luis Romanowski, Víctor
author_facet	Masson, Tomás Fabre, María Laura Ferrelli, María Leticia Pidre, Matías Luis Romanowski, Víctor
author_sort	Masson, Tomás
title	Protein composition of the occlusion bodies of <i>Epinotia aporema</i> granulovirus
title_short	Protein composition of the occlusion bodies of <i>Epinotia aporema</i> granulovirus
title_full	Protein composition of the occlusion bodies of <i>Epinotia aporema</i> granulovirus
title_fullStr	Protein composition of the occlusion bodies of <i>Epinotia aporema</i> granulovirus
title_full_unstemmed	Protein composition of the occlusion bodies of <i>Epinotia aporema</i> granulovirus
title_sort	protein composition of the occlusion bodies of <i>epinotia aporema</i> granulovirus
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/107807 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6372164&blobtype=pdf https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207735
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bdutipo_str	Repositorios
_version_	1764820443637743621

Protein composition of the occlusion bodies of <i>Epinotia aporema</i> granulovirus

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