Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

The apoplast, i.e. the cellular compartment external to the plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar to the apoplast alkalizati...

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Detalles Bibliográficos
Autores principales:	Borniego, María Lucía, Molinari, María Claudia, Guiamet, Juan José, Martínez, Dana Ethel
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2019
Materias:	Ciencias Naturales Apoplast Senescence Apoplastic fluid Secretome Extracellular pH Pathogenesis-related protein
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107736 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6960232&blobtype=pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107736
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Apoplast Senescence Apoplastic fluid Secretome Extracellular pH Pathogenesis-related protein
spellingShingle	Ciencias Naturales Apoplast Senescence Apoplastic fluid Secretome Extracellular pH Pathogenesis-related protein Borniego, María Lucía Molinari, María Claudia Guiamet, Juan José Martínez, Dana Ethel Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
topic_facet	Ciencias Naturales Apoplast Senescence Apoplastic fluid Secretome Extracellular pH Pathogenesis-related protein
description	The apoplast, i.e. the cellular compartment external to the plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar to the apoplast alkalization in response to abiotic stresses. The levels of 159 proteins decrease, whereas 24 proteins increase in relative abundance in the apoplast of senescing leaves. Around half of the apoplastic proteins of non-senescent leaves contain a N-terminal signal peptide for secretion, while all the identified senescence-associated apoplastic proteins contain the signal peptide. Several of the apoplastic proteins that accumulate during senescence also accumulate in stress responses, suggesting that the apoplast may constitute a compartment where developmental and stress-related programs overlap. Other senescence-related apoplastic proteins are involved in cell wall modifications, proteolysis, carbohydrate, ROS and amino acid metabolism, signaling, lipid transport, etc. The most abundant senescence-associated apoplastic proteins, PR2 and PR5 (e.g. pathogenesis related proteins PR2 and PR5) are related to leaf aging rather than to the chloroplast degradation program, as their levels increase only in leaves undergoing developmental senescence, but not in dark-induced senescent leaves. Changes in the apoplastic space may be relevant for signaling and molecular trafficking underlying senescence.
format	Articulo Articulo
author	Borniego, María Lucía Molinari, María Claudia Guiamet, Juan José Martínez, Dana Ethel
author_facet	Borniego, María Lucía Molinari, María Claudia Guiamet, Juan José Martínez, Dana Ethel
author_sort	Borniego, María Lucía
title	Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_short	Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_full	Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_fullStr	Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_full_unstemmed	Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_sort	physiological and proteomic changes in the apoplast accompany leaf senescence in arabidopsis
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/107736 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6960232&blobtype=pdf
work_keys_str_mv	AT borniegomarialucia physiologicalandproteomicchangesintheapoplastaccompanyleafsenescenceinarabidopsis AT molinarimariaclaudia physiologicalandproteomicchangesintheapoplastaccompanyleafsenescenceinarabidopsis AT guiametjuanjose physiologicalandproteomicchangesintheapoplastaccompanyleafsenescenceinarabidopsis AT martinezdanaethel physiologicalandproteomicchangesintheapoplastaccompanyleafsenescenceinarabidopsis
bdutipo_str	Repositorios
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Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

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