Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay

We describe the isolation and characterization of alfalfa-nodulating rhizobia from acid soils of different locations in Central Argentina and Uruguay. A collection of 465 isolates was assembled, and the rhizobia were characterized for acid tolerance. Growth tests revealed the existence of 15 acid-to...

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Autores principales: Del Papa, María Florencia, Balagué, Laura Josefina, Castro Sowinski, Susana, Wegener, Caren, Segundo, Eduardo, Martínez Abarca, Francisco, Toro, Nicolás, Niehaus, Karsten, Pühler, Alfred, Aguilar, Orlando Mario, Martinez-Drets, Gloria, Lagares, Antonio
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Biología
Bioquímica
Alfalfa-nodulating rhizobia
Acidic soils
AT strain Or191
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107702
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC91201&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107702
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bioquímica
Alfalfa-nodulating rhizobia
Acidic soils
AT strain Or191
spellingShingle Biología
Bioquímica
Alfalfa-nodulating rhizobia
Acidic soils
AT strain Or191
Del Papa, María Florencia
Balagué, Laura Josefina
Castro Sowinski, Susana
Wegener, Caren
Segundo, Eduardo
Martínez Abarca, Francisco
Toro, Nicolás
Niehaus, Karsten
Pühler, Alfred
Aguilar, Orlando Mario
Martinez-Drets, Gloria
Lagares, Antonio
Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay
topic_facet Biología
Bioquímica
Alfalfa-nodulating rhizobia
Acidic soils
AT strain Or191
description We describe the isolation and characterization of alfalfa-nodulating rhizobia from acid soils of different locations in Central Argentina and Uruguay. A collection of 465 isolates was assembled, and the rhizobia were characterized for acid tolerance. Growth tests revealed the existence of 15 acid-tolerant (AT) isolates which were able to grow at pH 5.0 and formed nodules in alfalfa with a low rate of nitrogen fixation. Analysis of those isolates, including partial sequencing of the genes encoding 16S rRNA and genomic PCR-fingerprinting with MBOREP1 and BOXC1 primers, demonstrated that the new isolates share a genetic background closely related to that of the previously reported Rhizobium sp. Or191 recovered from an acid soil in Oregon (B. D. Eardly, J. P. Young, and R. K. Selander, Appl. Environ. Microbiol. 58:1809–1815, 1992). Growth curves, melanin production, temperature tolerance, and megaplasmid profiles of the AT isolates were all coincident with these characteristics in strain Or191. In addition to the ability of all of these strains to nodulate alfalfa (Medicago sativa) inefficiently, the AT isolates also nodulated the common bean and Leucaena leucocephala, showing an extended host range for nodulation of legumes. In alfalfa, the time course of nodule formation by the AT isolate LPU 83 showed a continued nodulation restricted to the emerging secondary roots, which was probably related to the low rate of nitrogen fixation by the largely ineffective nodules. Results demonstrate the complexity of the rhizobial populations present in the acidic soils represented by a main group of N2-fixing rhizobia and a second group of ineffective and less-predominant isolates related to the AT strain Or191.
format Articulo
Articulo
author Del Papa, María Florencia
Balagué, Laura Josefina
Castro Sowinski, Susana
Wegener, Caren
Segundo, Eduardo
Martínez Abarca, Francisco
Toro, Nicolás
Niehaus, Karsten
Pühler, Alfred
Aguilar, Orlando Mario
Martinez-Drets, Gloria
Lagares, Antonio
author_facet Del Papa, María Florencia
Balagué, Laura Josefina
Castro Sowinski, Susana
Wegener, Caren
Segundo, Eduardo
Martínez Abarca, Francisco
Toro, Nicolás
Niehaus, Karsten
Pühler, Alfred
Aguilar, Orlando Mario
Martinez-Drets, Gloria
Lagares, Antonio
author_sort Del Papa, María Florencia
title Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay
title_short Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay
title_full Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay
title_fullStr Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay
title_full_unstemmed Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay
title_sort isolation and characterization of alfalfa-nodulating rhizobia present in acidic soils of central argentina and uruguay
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/107702
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC91201&blobtype=pdf
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