Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors

Extreme acidophiles play central roles in the geochemical cycling of diverse elements in low pH environments. This has been harnessed in biotechnologies such as biomining, where microorganisms facilitate the recovery of economically important metals such as gold. By generating both extreme acidity a...

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Autores principales: Ulloa, Ricardo, Moya Beltrán, Ana, Rojas Villalobos, Camila, Nuñez, Harold, Chiacchiarini, Patricia, Donati, Edgardo Rubén, Giaveno, Alejandra, Quatrini, Raquel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Exactas
Química
Biología
Acidithiobacillus
acidophiles
domestication
adaptation
consortia
targeted metagenomics
metagenome derived assembly
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107699
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6363673&blobtype=pdf
https://www.frontiersin.org/articles/10.3389/fmicb.2019.00060/full
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107699
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Biología
Acidithiobacillus
acidophiles
domestication
adaptation
consortia
targeted metagenomics
metagenome derived assembly
spellingShingle Ciencias Exactas
Química
Biología
Acidithiobacillus
acidophiles
domestication
adaptation
consortia
targeted metagenomics
metagenome derived assembly
Ulloa, Ricardo
Moya Beltrán, Ana
Rojas Villalobos, Camila
Nuñez, Harold
Chiacchiarini, Patricia
Donati, Edgardo Rubén
Giaveno, Alejandra
Quatrini, Raquel
Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors
topic_facet Ciencias Exactas
Química
Biología
Acidithiobacillus
acidophiles
domestication
adaptation
consortia
targeted metagenomics
metagenome derived assembly
description Extreme acidophiles play central roles in the geochemical cycling of diverse elements in low pH environments. This has been harnessed in biotechnologies such as biomining, where microorganisms facilitate the recovery of economically important metals such as gold. By generating both extreme acidity and a chemical oxidant (ferric iron) many species of prokaryotes that thrive in low pH environments not only catalyze mineral dissolution but also trigger both community and individual level adaptive changes. These changes vary in extent and direction depending on the ore mineralogy, water availability and local climate. The use of indigenous versus introduced microbial consortia in biomining practices is still a matter of debate. Yet, indigenous microbial consortia colonizing sulfidic ores that have been domesticated, i.e., selected for their ability to survive under specific polyextreme conditions, are claimed to outperform un-adapted foreign consortia. Despite this, little is known on the domestication of acidic microbial communities and the changes elicited in their members. In this study, high resolution targeted metagenomic techniques were used to analyze the changes occurring in the community structure of local microbial consortia acclimated to growing under extreme acidic conditions and adapted to endure the conditions imposed by the target mineral during biooxidation of a gold concentrate in an airlift reactor over a period of 2 years. The results indicated that operative conditions evolving through biooxidation of the mineral concentrate exerted strong selective pressures that, early on, purge biodiversity in favor of a few <i>Acidithiobacillus</i> spp. over other iron oxidizing acidophiles. Metagenomic analysis of the domesticated consortium present at the end of the adaptation experiment enabled reconstruction of the RVS1-MAG, a novel representative of <i>Acidithiobacillus ferrooxidans</i> from the Andacollo gold mineral district. Comparative genomic analysis performed with this genome draft revealed a net enrichment of gene functions related to heavy metal transport and stress management that are likely to play a significant role in adaptation and survival to adverse conditions experienced by these acidophiles during growth in presence of gold concentrates.
format Articulo
Articulo
author Ulloa, Ricardo
Moya Beltrán, Ana
Rojas Villalobos, Camila
Nuñez, Harold
Chiacchiarini, Patricia
Donati, Edgardo Rubén
Giaveno, Alejandra
Quatrini, Raquel
author_facet Ulloa, Ricardo
Moya Beltrán, Ana
Rojas Villalobos, Camila
Nuñez, Harold
Chiacchiarini, Patricia
Donati, Edgardo Rubén
Giaveno, Alejandra
Quatrini, Raquel
author_sort Ulloa, Ricardo
title Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors
title_short Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors
title_full Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors
title_fullStr Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors
title_full_unstemmed Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors
title_sort domestication of local microbial consortia for efficient recovery of gold through top-down selection in airlift bioreactors
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/107699
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6363673&blobtype=pdf
https://www.frontiersin.org/articles/10.3389/fmicb.2019.00060/full
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