Identification of cross-reactive B-cell epitopes between Bos d 9.0101(<i>Bos Taurus</i>) and Gly m 5.0101 (<i>Glycine max</i>) by epitope mapping MALDI-TOF MS

Exposure to cow’smilk constitutes one of the most common causes of food allergy. In addition, exposure to soy proteins has become relevant in a restricted proportion ofmilk allergic pediatric patients treated with soy formulae as a dairy substitute, because of the cross-allergenicity described betwe...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Candreva, Ángela María, Ferrer Navarro, Mario, Bronsoms, Silvia, Quiroga, Alejandra Viviana, Curciarello, Renata, Cauerhff, Ana Albina, Petruccelli, Silvana, Docena, Guillermo Horacio, Trejo, Sebastián Alejandro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Biología
Bos d 9.0101
cow’s milk
cross-reactivity
epitope mapping
Gly m 5.0101
MALDI-TOF MS
soybean
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107570
https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201700069
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107570
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Bos d 9.0101
cow’s milk
cross-reactivity
epitope mapping
Gly m 5.0101
MALDI-TOF MS
soybean
spellingShingle Ciencias Exactas
Biología
Bos d 9.0101
cow’s milk
cross-reactivity
epitope mapping
Gly m 5.0101
MALDI-TOF MS
soybean
Candreva, Ángela María
Ferrer Navarro, Mario
Bronsoms, Silvia
Quiroga, Alejandra Viviana
Curciarello, Renata
Cauerhff, Ana Albina
Petruccelli, Silvana
Docena, Guillermo Horacio
Trejo, Sebastián Alejandro
Identification of cross-reactive B-cell epitopes between Bos d 9.0101(<i>Bos Taurus</i>) and Gly m 5.0101 (<i>Glycine max</i>) by epitope mapping MALDI-TOF MS
topic_facet Ciencias Exactas
Biología
Bos d 9.0101
cow’s milk
cross-reactivity
epitope mapping
Gly m 5.0101
MALDI-TOF MS
soybean
description Exposure to cow’smilk constitutes one of the most common causes of food allergy. In addition, exposure to soy proteins has become relevant in a restricted proportion ofmilk allergic pediatric patients treated with soy formulae as a dairy substitute, because of the cross-allergenicity described between soy and milk proteins. We have previously identified several cross-reactive allergens between milk and soy that may explain this intolerance. The purpose of the present work was to identify epitopes in the purified αS1-casein and the recombinant soy allergen Gly m 5.0101 (Gly m 5) using an α-casein-specific monoclonal antibody (1D5 mAb) through two different approaches for epitope mapping, to understand cross-reactivity between milk and soy. The 1D5 mAb was immobilized onto magnetic beads, incubated with the peptide mixture previously obtained by enzymatic digestion of the allergens, and the captured peptides were identified by MALDI-TOFMS analysis. On a second approach, the peptidemixture was resolved by RP-HPLC and immunodominant peptides were identified by dot blot with the mAb. Finally, recognized peptides were sequenced by MALDI-TOF MS. This novel MS based approach led us to identify and characterize four peptides on α-casein and three peptides on Gly m 5 with a common core motif. Information obtained from these cross-reactive epitopes allows us to gain valuable insight into the molecular mechanisms of cross-reactivity, to further develop new and more effective vaccines for food allergy.
format Articulo
Articulo
author Candreva, Ángela María
Ferrer Navarro, Mario
Bronsoms, Silvia
Quiroga, Alejandra Viviana
Curciarello, Renata
Cauerhff, Ana Albina
Petruccelli, Silvana
Docena, Guillermo Horacio
Trejo, Sebastián Alejandro
author_facet Candreva, Ángela María
Ferrer Navarro, Mario
Bronsoms, Silvia
Quiroga, Alejandra Viviana
Curciarello, Renata
Cauerhff, Ana Albina
Petruccelli, Silvana
Docena, Guillermo Horacio
Trejo, Sebastián Alejandro
author_sort Candreva, Ángela María
title Identification of cross-reactive B-cell epitopes between Bos d 9.0101(<i>Bos Taurus</i>) and Gly m 5.0101 (<i>Glycine max</i>) by epitope mapping MALDI-TOF MS
title_short Identification of cross-reactive B-cell epitopes between Bos d 9.0101(<i>Bos Taurus</i>) and Gly m 5.0101 (<i>Glycine max</i>) by epitope mapping MALDI-TOF MS
title_full Identification of cross-reactive B-cell epitopes between Bos d 9.0101(<i>Bos Taurus</i>) and Gly m 5.0101 (<i>Glycine max</i>) by epitope mapping MALDI-TOF MS
title_fullStr Identification of cross-reactive B-cell epitopes between Bos d 9.0101(<i>Bos Taurus</i>) and Gly m 5.0101 (<i>Glycine max</i>) by epitope mapping MALDI-TOF MS
title_full_unstemmed Identification of cross-reactive B-cell epitopes between Bos d 9.0101(<i>Bos Taurus</i>) and Gly m 5.0101 (<i>Glycine max</i>) by epitope mapping MALDI-TOF MS
title_sort identification of cross-reactive b-cell epitopes between bos d 9.0101(<i>bos taurus</i>) and gly m 5.0101 (<i>glycine max</i>) by epitope mapping maldi-tof ms
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/107570
https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201700069
work_keys_str_mv AT candrevaangelamaria identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT ferrernavarromario identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT bronsomssilvia identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT quirogaalejandraviviana identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT curciarellorenata identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT cauerhffanaalbina identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT petruccellisilvana identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT docenaguillermohoracio identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
AT trejosebastianalejandro identificationofcrossreactivebcellepitopesbetweenbosd90101ibostaurusiandglym50101iglycinemaxibyepitopemappingmalditofms
bdutipo_str Repositorios
_version_ 1764820443325267969

Ejemplares similares