Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy

Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmen...

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Autores principales: Cattelan, Natalia, Yantorno, Osvaldo Miguel, Deora, Rajendar
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Biofilm
Confocal microscopy
Bordetella pertussis
Whooping cough
Biofilm-structure
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107519
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6108551&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107519
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biofilm
Confocal microscopy
Bordetella pertussis
Whooping cough
Biofilm-structure
spellingShingle Biología
Biofilm
Confocal microscopy
Bordetella pertussis
Whooping cough
Biofilm-structure
Cattelan, Natalia
Yantorno, Osvaldo Miguel
Deora, Rajendar
Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
topic_facet Biología
Biofilm
Confocal microscopy
Bordetella pertussis
Whooping cough
Biofilm-structure
description Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host immune defenses. When in biofilms, the microbial cells interact with each other and with the surface to develop architecturally complex multidimensional structures. Numerous imaging techniques and tools are currently available for architectural analyses of biofilm communities. This allows examination of biofilm development through acquisition of three-dimensional images that can render structural features of the sessile community. A frequently utilized tool is Confocal Laser Scanning Microscopy. We present a detailed protocol to grow, observe and analyze biofilms of the respiratory human pathogen, Bordetella pertussis in space and time.
format Articulo
Preprint
author Cattelan, Natalia
Yantorno, Osvaldo Miguel
Deora, Rajendar
author_facet Cattelan, Natalia
Yantorno, Osvaldo Miguel
Deora, Rajendar
author_sort Cattelan, Natalia
title Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
title_short Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
title_full Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
title_fullStr Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
title_full_unstemmed Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
title_sort structural analysis of bordetella pertussis biofilms by confocal laser scanning microscopy
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/107519
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6108551&blobtype=pdf
work_keys_str_mv AT cattelannatalia structuralanalysisofbordetellapertussisbiofilmsbyconfocallaserscanningmicroscopy
AT yantornoosvaldomiguel structuralanalysisofbordetellapertussisbiofilmsbyconfocallaserscanningmicroscopy
AT deorarajendar structuralanalysisofbordetellapertussisbiofilmsbyconfocallaserscanningmicroscopy
bdutipo_str Repositorios
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