Single yeast cell nanomotions correlate with cellular activity

Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the...

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Detalles Bibliográficos
Autores principales: Willaert, Ronnie G, Vanden Boer, Pieterjan, Malovichko, Anton, Alioscha-Perez, Mitchel, Radotić, Ksenija, Bartolić, Dragana, Kalauzi, Aleksandar, Villalba, María Inés, Sanglard, Dominique, Dietler, Giovanni, Sahli, Hichem, Kasas, Sandor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107502
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7314535&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107502
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
spellingShingle Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
Willaert, Ronnie G
Vanden Boer, Pieterjan
Malovichko, Anton
Alioscha-Perez, Mitchel
Radotić, Ksenija
Bartolić, Dragana
Kalauzi, Aleksandar
Villalba, María Inés
Sanglard, Dominique
Dietler, Giovanni
Sahli, Hichem
Kasas, Sandor
Single yeast cell nanomotions correlate with cellular activity
topic_facet Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
description Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
format Articulo
Articulo
author Willaert, Ronnie G
Vanden Boer, Pieterjan
Malovichko, Anton
Alioscha-Perez, Mitchel
Radotić, Ksenija
Bartolić, Dragana
Kalauzi, Aleksandar
Villalba, María Inés
Sanglard, Dominique
Dietler, Giovanni
Sahli, Hichem
Kasas, Sandor
author_facet Willaert, Ronnie G
Vanden Boer, Pieterjan
Malovichko, Anton
Alioscha-Perez, Mitchel
Radotić, Ksenija
Bartolić, Dragana
Kalauzi, Aleksandar
Villalba, María Inés
Sanglard, Dominique
Dietler, Giovanni
Sahli, Hichem
Kasas, Sandor
author_sort Willaert, Ronnie G
title Single yeast cell nanomotions correlate with cellular activity
title_short Single yeast cell nanomotions correlate with cellular activity
title_full Single yeast cell nanomotions correlate with cellular activity
title_fullStr Single yeast cell nanomotions correlate with cellular activity
title_full_unstemmed Single yeast cell nanomotions correlate with cellular activity
title_sort single yeast cell nanomotions correlate with cellular activity
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/107502
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7314535&blobtype=pdf
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