Complete Genome Sequencing of <i>Acinetobacter baumannii</i> Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11

Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50, recovered from a hospitalized patient in Kuwait, exhibited resistance to carbapenems and additi...

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Autores principales: Wibberg, Daniel, Salto, Ileana Paula, Eikmeyer, Félix G., Maus, Irena, Winkler, Anika, Nordmann, Patrice, Pühler, Alfred, Poirel, Laurent, Schlüter, Andreas
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
antibiotic resistance
b-lactamase
carbapenemase
conjugative transfer
Acinetobacter baumannii
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107106
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5923157&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107106
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
antibiotic resistance
b-lactamase
carbapenemase
conjugative transfer
Acinetobacter baumannii
spellingShingle Ciencias Exactas
antibiotic resistance
b-lactamase
carbapenemase
conjugative transfer
Acinetobacter baumannii
Wibberg, Daniel
Salto, Ileana Paula
Eikmeyer, Félix G.
Maus, Irena
Winkler, Anika
Nordmann, Patrice
Pühler, Alfred
Poirel, Laurent
Schlüter, Andreas
Complete Genome Sequencing of <i>Acinetobacter baumannii</i> Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11
topic_facet Ciencias Exactas
antibiotic resistance
b-lactamase
carbapenemase
conjugative transfer
Acinetobacter baumannii
description Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50, recovered from a hospitalized patient in Kuwait, exhibited resistance to carbapenems and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin, and gentamicin. Genome sequencing revealed that the strain possesses two plasmids, pK50a (79.6 kb) and pK50b (9.5 kb), and a 3.75-Mb chromosome. A. baumannii K50 exhibits an average nucleotide identity (ANI) of 99.98% to the previously reported Iraqi clinical isolate AA-014, even though the latter strain lacked plasmid pK50a. Strain K50 belongs to sequence type 158 (ST158) (Pasteur scheme) and ST499 (Oxford scheme). Plasmid pK50a is a member of the Aci6 (replication group 6 [RG6]) group of Acinetobacter plasmids and carries a conjugative transfer module and two antibiotic resistance gene regions. The transposon Tn2008 carries the carbapenemase gene blaOXA-23, whereas a class 1 integron harbors the resistance genes blaGES-11, aacA4, dfrA7, qacEΔ1, and sul1, conferring resistance to all b-lactams and reduced susceptibility to carbapenems and resistance to aminoglycosides, trimethoprim, quaternary ammonium compounds, and sulfamethoxazole, respectively. The class 1 integron is flanked by MITEs (miniature inverted-repeat transposable elements) delimiting the element at its insertion site.
format Articulo
Articulo
author Wibberg, Daniel
Salto, Ileana Paula
Eikmeyer, Félix G.
Maus, Irena
Winkler, Anika
Nordmann, Patrice
Pühler, Alfred
Poirel, Laurent
Schlüter, Andreas
author_facet Wibberg, Daniel
Salto, Ileana Paula
Eikmeyer, Félix G.
Maus, Irena
Winkler, Anika
Nordmann, Patrice
Pühler, Alfred
Poirel, Laurent
Schlüter, Andreas
author_sort Wibberg, Daniel
title Complete Genome Sequencing of <i>Acinetobacter baumannii</i> Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11
title_short Complete Genome Sequencing of <i>Acinetobacter baumannii</i> Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11
title_full Complete Genome Sequencing of <i>Acinetobacter baumannii</i> Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11
title_fullStr Complete Genome Sequencing of <i>Acinetobacter baumannii</i> Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11
title_full_unstemmed Complete Genome Sequencing of <i>Acinetobacter baumannii</i> Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11
title_sort complete genome sequencing of <i>acinetobacter baumannii</i> strain k50 discloses the large conjugative plasmid pk50a encoding carbapenemase oxa-23 and extended-spectrum β-lactamase ges-11
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/107106
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5923157&blobtype=pdf
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