Selección de cepas de levaduras productoras de enzimas pécticas
The present study was undertaken to find yeast strains with high pectinolytic activity. Yeast strains isolated from citrus fruits, were screened for pectinase production. The species with the best pectinase activity was selected to evaluate their ability to produce pectic enzymes by submerged fermen...
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Autores principales: | , , , , , |
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Formato: | Articulo |
Lenguaje: | Español |
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2007
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/106032 https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/592 |
Aporte de: |
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I19-R120-10915-106032 |
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dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Español |
topic |
Biología Química Geotrichum sp. Screening of yeasts Polygalacturonase Maceration Selección de levaduras Poligalacturonasa Maceración |
spellingShingle |
Biología Química Geotrichum sp. Screening of yeasts Polygalacturonase Maceration Selección de levaduras Poligalacturonasa Maceración Martos, María Alicia Zubreski, Emilce R. Velázquez, Gabriela S. Martínez Vázquez, Francisco Benassi, Fernando O. Hours, Roque Alberto Selección de cepas de levaduras productoras de enzimas pécticas |
topic_facet |
Biología Química Geotrichum sp. Screening of yeasts Polygalacturonase Maceration Selección de levaduras Poligalacturonasa Maceración |
description |
The present study was undertaken to find yeast strains with high pectinolytic activity. Yeast strains isolated from citrus fruits, were screened for pectinase production. The species with the best pectinase activity was selected to evaluate their ability to produce pectic enzymes by submerged fermentation processes. The effect of different carbon sources on polygalacturonase (PGase) production was studied. Different vegetable tissues were used for tests of maceration with the crude enzyme extract.
Among 154 yeast strains isolated, yeast Nº 111 was positive for pectinase activity and was identified as Geotrichum sp. Maximum PGase production obtained was 90.7 EU/ml when this yeast grown in shake flasks under the presence of glucose and citrus pectin as carbon sources. All tissues studied were macerated by the enzyme extract, microscopic examination showed a suspension of loose single cells. Lysis of cells was not observed. |
format |
Articulo Articulo |
author |
Martos, María Alicia Zubreski, Emilce R. Velázquez, Gabriela S. Martínez Vázquez, Francisco Benassi, Fernando O. Hours, Roque Alberto |
author_facet |
Martos, María Alicia Zubreski, Emilce R. Velázquez, Gabriela S. Martínez Vázquez, Francisco Benassi, Fernando O. Hours, Roque Alberto |
author_sort |
Martos, María Alicia |
title |
Selección de cepas de levaduras productoras de enzimas pécticas |
title_short |
Selección de cepas de levaduras productoras de enzimas pécticas |
title_full |
Selección de cepas de levaduras productoras de enzimas pécticas |
title_fullStr |
Selección de cepas de levaduras productoras de enzimas pécticas |
title_full_unstemmed |
Selección de cepas de levaduras productoras de enzimas pécticas |
title_sort |
selección de cepas de levaduras productoras de enzimas pécticas |
publishDate |
2007 |
url |
http://sedici.unlp.edu.ar/handle/10915/106032 https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/592 |
work_keys_str_mv |
AT martosmariaalicia selecciondecepasdelevadurasproductorasdeenzimaspecticas AT zubreskiemilcer selecciondecepasdelevadurasproductorasdeenzimaspecticas AT velazquezgabrielas selecciondecepasdelevadurasproductorasdeenzimaspecticas AT martinezvazquezfrancisco selecciondecepasdelevadurasproductorasdeenzimaspecticas AT benassifernandoo selecciondecepasdelevadurasproductorasdeenzimaspecticas AT hoursroquealberto selecciondecepasdelevadurasproductorasdeenzimaspecticas AT martosmariaalicia screeningofyeaststrainswithpectinolyticactivity AT zubreskiemilcer screeningofyeaststrainswithpectinolyticactivity AT velazquezgabrielas screeningofyeaststrainswithpectinolyticactivity AT martinezvazquezfrancisco screeningofyeaststrainswithpectinolyticactivity AT benassifernandoo screeningofyeaststrainswithpectinolyticactivity AT hoursroquealberto screeningofyeaststrainswithpectinolyticactivity |
bdutipo_str |
Repositorios |
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1764820442922614785 |