Bioprocessing of “Hair Waste” by <i>Paecilomyces lilacinus</i> as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis
<i>Paecilomyces lilacinus</i> (Thom) Samson LPS 876, a locally isolated fungal strain, was grown on minimal mineral medium containing “hair waste,” a residue from the hair-saving unhairing process, and produced a protease with keratinolytic activity. This enzyme was biochemically charact...
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| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2012
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/104780 http://hdl.handle.net/11336/93905 https://www.hindawi.com/journals/btri/2012/369308/ |
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I19-R120-10915-104780 |
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dspace |
| institution |
Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas hair waste detergent stable serine proteases keratinolytic activity thermostability |
| spellingShingle |
Ciencias Exactas hair waste detergent stable serine proteases keratinolytic activity thermostability Cavello, Ivana Alejandra Hours, Roque Alberto Cavalitto, Sebastián Fernando Bioprocessing of “Hair Waste” by <i>Paecilomyces lilacinus</i> as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis |
| topic_facet |
Ciencias Exactas hair waste detergent stable serine proteases keratinolytic activity thermostability |
| description |
<i>Paecilomyces lilacinus</i> (Thom) Samson LPS 876, a locally isolated fungal strain, was grown on minimal mineral medium containing “hair waste,” a residue from the hair-saving unhairing process, and produced a protease with keratinolytic activity. This enzyme was biochemically characterized. The optimum reaction conditions, determined with a response surface methodology, were 60°C and pH 6.0. It was remarkably stable in a wide range of pHs and temperatures. Addition of Ca<sup>2+</sup>, Mg<sup>2+</sup>, or sorbitol was found to be effective in increasing thermal stability of the protease. PMSF and Hg<sup>2+</sup> inhibited the proteolytic activity indicating the presence of a thiol-dependent serine protease. It showed high stability toward surfactants, bleaching agents, and solvents. It was also compatible with commercial detergents (7 mg/mL) such as Ariel, Skip, Drive, and Ace, retaining more than 70% of its proteolytic activity in all detergents after 1 h of incubation at 40°C. Wash performance analysis revealed that this protease could effectively remove blood stains. From these properties, this enzyme may be considered as a potential candidate for future use in biotechnological processes, as well as in the formulation of laundry detergents. |
| format |
Articulo Articulo |
| author |
Cavello, Ivana Alejandra Hours, Roque Alberto Cavalitto, Sebastián Fernando |
| author_facet |
Cavello, Ivana Alejandra Hours, Roque Alberto Cavalitto, Sebastián Fernando |
| author_sort |
Cavello, Ivana Alejandra |
| title |
Bioprocessing of “Hair Waste” by <i>Paecilomyces lilacinus</i> as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis |
| title_short |
Bioprocessing of “Hair Waste” by <i>Paecilomyces lilacinus</i> as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis |
| title_full |
Bioprocessing of “Hair Waste” by <i>Paecilomyces lilacinus</i> as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis |
| title_fullStr |
Bioprocessing of “Hair Waste” by <i>Paecilomyces lilacinus</i> as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis |
| title_full_unstemmed |
Bioprocessing of “Hair Waste” by <i>Paecilomyces lilacinus</i> as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis |
| title_sort |
bioprocessing of “hair waste” by <i>paecilomyces lilacinus</i> as a source of a bleach-stable, alkaline, and thermostable keratinase with potential application as a laundry detergent additive: characterization and wash performance analysis |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/104780 http://hdl.handle.net/11336/93905 https://www.hindawi.com/journals/btri/2012/369308/ |
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Repositorios |
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