Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria

An efficient and green bioprocess is herein reported to obtain halogenated nucleosides by transglycosylation using immobilized lactic acid bacteria (LAB). Lactobacillus animalis ATCC 35046 showed a yield of 95% at 0.5 h to synthesize 5-fluorouracil-2′-deoxyriboside (floxuridine). Calcium alginate wa...

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Detalles Bibliográficos
Autores principales: Britos, Claudia Noelia, Cappa, Valeria Alejandra, Rivero, Cintia Wanda, Sambeth, Jorge Enrique, Lozano, Mario Enrique, Trelles, Jorge Abel
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2012
Materias:
Química
Bioquímica
Lactic acid bacteria
2´-N-deoxyribosyltransferase
Whole-cell immobilization
Antitumoral compounds
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104350
http://hdl.handle.net/11336/36731
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104350
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Bioquímica
Lactic acid bacteria
2´-N-deoxyribosyltransferase
Whole-cell immobilization
Antitumoral compounds
spellingShingle Química
Bioquímica
Lactic acid bacteria
2´-N-deoxyribosyltransferase
Whole-cell immobilization
Antitumoral compounds
Britos, Claudia Noelia
Cappa, Valeria Alejandra
Rivero, Cintia Wanda
Sambeth, Jorge Enrique
Lozano, Mario Enrique
Trelles, Jorge Abel
Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
topic_facet Química
Bioquímica
Lactic acid bacteria
2´-N-deoxyribosyltransferase
Whole-cell immobilization
Antitumoral compounds
description An efficient and green bioprocess is herein reported to obtain halogenated nucleosides by transglycosylation using immobilized lactic acid bacteria (LAB). Lactobacillus animalis ATCC 35046 showed a yield of 95% at 0.5 h to synthesize 5-fluorouracil-2′-deoxyriboside (floxuridine). Calcium alginate was the best matrix for whole-cell immobilization by entrapment. Its productivity was 87 mg/L h in a continuous bioprocess. When adsorption techniques were evaluated, DEAE-Sepharose was the support which showed higher microbial load, its productivity being 53 mg/L h. Additionally, this microorganism was able to produce 5-bromouracil-2′-deoxyriboside, 6-chloropurine-2′-deoxyriboside and 6-bromopurine-2′-deoxyriboside.
format Articulo
Preprint
author Britos, Claudia Noelia
Cappa, Valeria Alejandra
Rivero, Cintia Wanda
Sambeth, Jorge Enrique
Lozano, Mario Enrique
Trelles, Jorge Abel
author_facet Britos, Claudia Noelia
Cappa, Valeria Alejandra
Rivero, Cintia Wanda
Sambeth, Jorge Enrique
Lozano, Mario Enrique
Trelles, Jorge Abel
author_sort Britos, Claudia Noelia
title Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
title_short Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
title_full Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
title_fullStr Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
title_full_unstemmed Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
title_sort biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/104350
http://hdl.handle.net/11336/36731
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