Endocytosis and intracellular traffic of cholesterol-PDMAEMA liposome complexes in human epithelial-like cells

Liposomes are generally used as delivery systems, as they are capable of encapsulating a wide variety of molecules (i.e. plasmids, recombinant proteins, therapeutic drugs). However, liposomal drug delivery have to fulfill different requirements, such as the effective internalization by the target ce...

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Detalles Bibliográficos
Autores principales: Szymanowski, Felipe, Hugo, Ayelén, Alves, P., Simões, P. N., Gómez-Zavaglia, Andrea, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Español
Publicado: 2017
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103896
Aporte de:
id I19-R120-10915-103896
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Química
CHO-PDMAEMA
Polymer-lipid complexes
Liposome internalization
Endocytic pathways
Caveolae
spellingShingle Química
CHO-PDMAEMA
Polymer-lipid complexes
Liposome internalization
Endocytic pathways
Caveolae
Szymanowski, Felipe
Hugo, Ayelén
Alves, P.
Simões, P. N.
Gómez-Zavaglia, Andrea
Pérez, Pablo Fernando
Endocytosis and intracellular traffic of cholesterol-PDMAEMA liposome complexes in human epithelial-like cells
topic_facet Química
CHO-PDMAEMA
Polymer-lipid complexes
Liposome internalization
Endocytic pathways
Caveolae
description Liposomes are generally used as delivery systems, as they are capable of encapsulating a wide variety of molecules (i.e. plasmids, recombinant proteins, therapeutic drugs). However, liposomal drug delivery have to fulfill different requirements, such as the effective internalization by the target cells and avoidance of the degradative activity of the intracellular compartments. The use of polymer lipid complexes (PLCs), by including different polymers in the liposome formulation, could improve internalization and intracellular release of drugs. The aim of the present work is to study the mechanisms of cellular uptaking and the intracellular trafficking of PLCs formed with cholesterol-poly(2-(dimethylamino)ethyl methacrylate) CHO-PDMAEMA and lecithin (LC CHO-PD). Calcein-loaded liposomes were used to determine cellular uptake and intracellular localization by flow cytometry and confocal microscopy. Incorporation of CHOPDMAEMA to lecithin liposomes enhanced the internalization capacity of PLCs. Internalization of PLCs by human epithelial-like cells (HEK-293) diminished at 4 ◦C, suggesting uptake by endocytosis. PLCs showed no co-localization with acidic compartments after internalization. Experiments with endocytosis inhibitors and co-localization of liposomes and albumin, suggested the caveolae endocytic pathway as the most probable route for intracellular trafficking of PLCs. In this work, we demonstrated an efficient uptake of LC CHO-PDs by human epithelial-like cells (HEK- 293) through the non-degradative caveolae endocytic pathway. The mode of internalization and the intracellular fate of liposomes under study, suggest a promising use of LC CHO-PDs as drug delivery systems.
format Articulo
Articulo
author Szymanowski, Felipe
Hugo, Ayelén
Alves, P.
Simões, P. N.
Gómez-Zavaglia, Andrea
Pérez, Pablo Fernando
author_facet Szymanowski, Felipe
Hugo, Ayelén
Alves, P.
Simões, P. N.
Gómez-Zavaglia, Andrea
Pérez, Pablo Fernando
author_sort Szymanowski, Felipe
title Endocytosis and intracellular traffic of cholesterol-PDMAEMA liposome complexes in human epithelial-like cells
title_short Endocytosis and intracellular traffic of cholesterol-PDMAEMA liposome complexes in human epithelial-like cells
title_full Endocytosis and intracellular traffic of cholesterol-PDMAEMA liposome complexes in human epithelial-like cells
title_fullStr Endocytosis and intracellular traffic of cholesterol-PDMAEMA liposome complexes in human epithelial-like cells
title_full_unstemmed Endocytosis and intracellular traffic of cholesterol-PDMAEMA liposome complexes in human epithelial-like cells
title_sort endocytosis and intracellular traffic of cholesterol-pdmaema liposome complexes in human epithelial-like cells
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103896
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