Effect of an <i>Ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved

Tea made from Ilex paraguariensis (IP) dried and minced leaves is a beverage widely consumed by large populations in South America as a source of caffeine (stimulant action) and for its medicinal properties. However, there is little information about the action of IP on the myocardium in the ischemi...

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Autores principales: González Arbeláez, Luisa Fernanda, Fantinelli, Juliana Catalina, Ciocci Pardo, Alejandro, Caldiz, Claudia Irma, Ríos, José Luis, Schinella, Guillermo Raúl, Mosca, Susana María
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Médicas
Ilex paraguariensis
ischemia-reperfusion
TBARS
GSH
P-Akt/P-eNOS
mPTP
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103863
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103863
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Ilex paraguariensis
ischemia-reperfusion
TBARS
GSH
P-Akt/P-eNOS
mPTP
spellingShingle Ciencias Médicas
Ilex paraguariensis
ischemia-reperfusion
TBARS
GSH
P-Akt/P-eNOS
mPTP
González Arbeláez, Luisa Fernanda
Fantinelli, Juliana Catalina
Ciocci Pardo, Alejandro
Caldiz, Claudia Irma
Ríos, José Luis
Schinella, Guillermo Raúl
Mosca, Susana María
Effect of an <i>Ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved
topic_facet Ciencias Médicas
Ilex paraguariensis
ischemia-reperfusion
TBARS
GSH
P-Akt/P-eNOS
mPTP
description Tea made from Ilex paraguariensis (IP) dried and minced leaves is a beverage widely consumed by large populations in South America as a source of caffeine (stimulant action) and for its medicinal properties. However, there is little information about the action of IP on the myocardium in the ischemia–reperfusion condition. Therefore, the objective of this study was to examine the effects of an aqueous extract of IP on infarct size in a model of regional ischemia. Isolated rat hearts were perfused by the Langendorff technique and subjected to 40 min of coronary artery occlusion followed by 60 min of reperfusion (ischemic control hearts). Other hearts received IP 30 μg mL−1 during the first 10 min of reperfusion in the absence or presence of LG-nitro-L-arginine methyl ester [L-NAME, a nitric oxide synthase (NOS) inhibitor]. The infarct size was determined by triphenyltetrazolium chloride (TTC) staining. Post-ischemic myocardial function and coronary perfusion were also assessed. Cardiac oxidative damage was evaluated by using the thiobarbituric acid reactive substance (TBARS) concentration and the reduced glutathione (GSH) content. To analyze the mechanisms involved, the expressions of phosphorylated forms of eNOS and Akt were measured. In isolated mitochondria the Ca2+-induced mitochondrial permeability transition pore (mPTP) opening was determined. IP significantly decreased the infarct size and improved post-ischemic myocardial function and coronary perfusion. TBARS decreased, GSH was partially preserved, the levels of P-eNOS and P-Akt increased and mPTP opening diminished after IP addition. These changes were abolished by L-NAME. Therefore, our data demonstrate that acute treatment with IP only during reperfusion was effective in reducing myocardial post-ischemic alterations. These actions would be mediated by a decrease of mitochondrial permeability through IP-activated Akt/eNOS-dependent pathways.
format Articulo
Preprint
author González Arbeláez, Luisa Fernanda
Fantinelli, Juliana Catalina
Ciocci Pardo, Alejandro
Caldiz, Claudia Irma
Ríos, José Luis
Schinella, Guillermo Raúl
Mosca, Susana María
author_facet González Arbeláez, Luisa Fernanda
Fantinelli, Juliana Catalina
Ciocci Pardo, Alejandro
Caldiz, Claudia Irma
Ríos, José Luis
Schinella, Guillermo Raúl
Mosca, Susana María
author_sort González Arbeláez, Luisa Fernanda
title Effect of an <i>Ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved
title_short Effect of an <i>Ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved
title_full Effect of an <i>Ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved
title_fullStr Effect of an <i>Ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved
title_full_unstemmed Effect of an <i>Ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved
title_sort effect of an <i>ilex paraguariensis</i> (yerba mate) extract on infarct size in isolated rat hearts: the mechanisms involved
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/103863
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