Design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy

Linalool (LN) is a monoterpene found in essential oils of plants and herbs that produces multiple effects on the mevalonate pathway and interesting antiproliferative activity in cancer cells. However, due to its poor aqueous solubility, an efficient vehicle is needed to improve its administration an...

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Detalles Bibliográficos
Autores principales: Rodenak-Kladniew, Boris, Islan, Germán Abel, García de Bravo, Margarita María, Durán, Nelson, Castro, Guillermo Raúl
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Linalool solid lipid nanoparticles
Cytotoxicity
Drug delivery
Cancer cells
Cellular uptake
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103740
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103740
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Linalool solid lipid nanoparticles
Cytotoxicity
Drug delivery
Cancer cells
Cellular uptake
spellingShingle Química
Linalool solid lipid nanoparticles
Cytotoxicity
Drug delivery
Cancer cells
Cellular uptake
Rodenak-Kladniew, Boris
Islan, Germán Abel
García de Bravo, Margarita María
Durán, Nelson
Castro, Guillermo Raúl
Design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy
topic_facet Química
Linalool solid lipid nanoparticles
Cytotoxicity
Drug delivery
Cancer cells
Cellular uptake
description Linalool (LN) is a monoterpene found in essential oils of plants and herbs that produces multiple effects on the mevalonate pathway and interesting antiproliferative activity in cancer cells. However, due to its poor aqueous solubility, an efficient vehicle is needed to improve its administration and bioavailability in physiological media. LN encapsulation in solid lipid nanoparticles (SLN) with different compositions was explored and in vitro tested in two cancer cell lines. SLN of myristyl myristate (MM), cetyl esters (SS) and cetyl palmitate (CP) were prepared by sonication in the presence of Pluronic®F68 as surfactant. Nanoparticle size, morphology and distribution were determined by dynamic light scattering in combination with optical and transmission electron microscopy (TEM). SLN showed spherical shape and mean diameters in the range of 90–130 nm with narrow size dispersion (PDI values lower than 0.2) and Z potentials around −4.0 mV. The encapsulation percentages of LN in SLN were higher than 80% for all tested formulations and exhibited in vitro LN controlled release profiles for at least 72 h. The nanoparticles were physicochemically characterized by FTIR, XRD, DSC and TGA, and the incorporation of LN into SLN was higher than 80% in tested matrices. The developed formulations, and in particular SLN (MM)-LN, showed in vitro antiproliferative effects on hepatocarcinoma (HepG2) and lung adenocarcinoma (A549) cell lines in a dose-dependent response, and higher inhibitory effects were found in comparison with free LN. The cellular uptake of SLN was demonstrated by fluorescence microscopy, enhancing the ability of nanoparticles to intracellularly deliver the cargo molecules.
format Articulo
Articulo
author Rodenak-Kladniew, Boris
Islan, Germán Abel
García de Bravo, Margarita María
Durán, Nelson
Castro, Guillermo Raúl
author_facet Rodenak-Kladniew, Boris
Islan, Germán Abel
García de Bravo, Margarita María
Durán, Nelson
Castro, Guillermo Raúl
author_sort Rodenak-Kladniew, Boris
title Design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy
title_short Design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy
title_full Design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy
title_fullStr Design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy
title_full_unstemmed Design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy
title_sort design, characterization and in vitro evaluation of linalool-loaded solid lipid nanoparticles as potent tool in cancer therapy
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103740
work_keys_str_mv AT rodenakkladniewboris designcharacterizationandinvitroevaluationoflinaloolloadedsolidlipidnanoparticlesaspotenttoolincancertherapy
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bdutipo_str Repositorios
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