A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis
A novel high performance system to control the temperature of the microcartridge in on-line solid phase extraction capillary electrophoresis (SPE–CE) is introduced. The mini-device consists in a thermostatic bath that fits inside of the cassette of any commercial CE instrument, while its temperature...
| Autores principales: | , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2015
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/103113 https://www.sciencedirect.com/science/article/abs/pii/S0003267014014913 |
| Aporte de: |
| id |
I19-R120-10915-103113 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Química Ingeniería Capillary electrophoresis On-line pre-concentration Opioid peptides Solid-Phase extraction Temperature |
| spellingShingle |
Ciencias Exactas Química Ingeniería Capillary electrophoresis On-line pre-concentration Opioid peptides Solid-Phase extraction Temperature Tascon, Marcos Benavente, Fernando Sanz Nebot, Victoria Gagliardi, Leonardo Gabriel A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis |
| topic_facet |
Ciencias Exactas Química Ingeniería Capillary electrophoresis On-line pre-concentration Opioid peptides Solid-Phase extraction Temperature |
| description |
A novel high performance system to control the temperature of the microcartridge in on-line solid phase extraction capillary electrophoresis (SPE–CE) is introduced. The mini-device consists in a thermostatic bath that fits inside of the cassette of any commercial CE instrument, while its temperature is controlled from an external circuit of liquid connecting three differentwater baths. The circuits are controlled from a switchboard connected to an array of electrovalves that allow to rapidly alternate the water circulation through the mini-thermostatic-bath between temperatures from 5 to 90 ºC. The combination of the mini-device and the forced-air thermostatization system of the commercial CE instrument allows to optimize independently the temperature of the sample loading, the clean-up, the analyte elution and the electrophoretic separation steps.
The system is used to study the effect of temperature on the C18-SPE–CE analysis of the opioid peptides, Dynorphin A (Dyn A), Endomorphin1 (END) and Met-enkephalin (MET), in both standard solutions and in spiked plasma samples. Extraction recoveries demonstrated to depend, with a non-monotonous trend, on the microcartridge temperature during the sample loading and became maximum at 60 ºC. Results prove the potential of temperature control to further enhance sensitivity in SPE–CE when analytes are thermally stable. |
| format |
Articulo Articulo |
| author |
Tascon, Marcos Benavente, Fernando Sanz Nebot, Victoria Gagliardi, Leonardo Gabriel |
| author_facet |
Tascon, Marcos Benavente, Fernando Sanz Nebot, Victoria Gagliardi, Leonardo Gabriel |
| author_sort |
Tascon, Marcos |
| title |
A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis |
| title_short |
A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis |
| title_full |
A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis |
| title_fullStr |
A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis |
| title_full_unstemmed |
A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis |
| title_sort |
high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis |
| publishDate |
2015 |
| url |
http://sedici.unlp.edu.ar/handle/10915/103113 https://www.sciencedirect.com/science/article/abs/pii/S0003267014014913 |
| work_keys_str_mv |
AT tasconmarcos ahighperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis AT benaventefernando ahighperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis AT sanznebotvictoria ahighperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis AT gagliardileonardogabriel ahighperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis AT tasconmarcos highperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis AT benaventefernando highperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis AT sanznebotvictoria highperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis AT gagliardileonardogabriel highperformancesystemtostudytheinfluenceoftemperatureinonlinesolidphaseextractioncapillaryelectrophoresis |
| bdutipo_str |
Repositorios |
| _version_ |
1764820441520668672 |