Lipase B of <i>Candida antarctica</i> co-adsorbed with polyols onto TiO<sub>2</sub> nanoparticles for improved biocatalytic performance

<b>Background</b>: The immobilization of the lipase B of <i>Candida antarctica</i> CALB over TiO<sub>2</sub> nanoparticles was thoroughly investigated with the isotherms of adsorption at various temperatures with and without the addition of sorbitol and glycerol....

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Detalles Bibliográficos
Autores principales: Llerena Suster, Carlos Rafael, Toledo, María Victoria, Fittipaldi, Antonela Soledad, Morcelle del Valle, Susana Raquel, Briand, Laura Estefanía
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Ciencias Exactas
Adsorption
Lipases
Biocatalysis
Immobilization
Candida antarctica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103071
https://onlinelibrary.wiley.com/doi/10.1002/jctb.5305
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103071
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Exactas
Adsorption
Lipases
Biocatalysis
Immobilization
Candida antarctica
spellingShingle Biología
Ciencias Exactas
Adsorption
Lipases
Biocatalysis
Immobilization
Candida antarctica
Llerena Suster, Carlos Rafael
Toledo, María Victoria
Fittipaldi, Antonela Soledad
Morcelle del Valle, Susana Raquel
Briand, Laura Estefanía
Lipase B of <i>Candida antarctica</i> co-adsorbed with polyols onto TiO<sub>2</sub> nanoparticles for improved biocatalytic performance
topic_facet Biología
Ciencias Exactas
Adsorption
Lipases
Biocatalysis
Immobilization
Candida antarctica
description <b>Background</b>: The immobilization of the lipase B of <i>Candida antarctica</i> CALB over TiO<sub>2</sub> nanoparticles was thoroughly investigated with the isotherms of adsorption at various temperatures with and without the addition of sorbitol and glycerol. The surface composition, secondary structure and the effect of the addition of the polyols was addressed. <b>Results</b>: The maximum dispersion limit of protein on TiO<sub>2</sub> nanoparticles (NPs) is 0.073 ± 0.007 µmol m<sup>−2</sup>. Glycerol and sorbitol co‐adsorb on the TiO<sub>2</sub> NPs reaching 45% of the surface composition of the biocatalyst. The optimized material was able to catalyze the esterification of 52% of R/S‐ibuprofen with ethanol (0.31 ± 0.01 µmol min<sup>−1</sup> mg<sup>−1</sup>) with 41% of enantiomeric excess towards S(+)‐ibuprofen in 24 h reaction. Under similar reaction conditions, the commercial counterpart Novozym® 435 showed 34% conversion (0.091 ± 0.003 µmol min<sup>−1</sup> mg<sup>−1</sup>) and 16% of enantiomeric excess. <b>Conclusion</b>: The molecular association between the protein and the polyols exerts a positive cooperativism which prevents aggregation of the protein and protects its active conformation. The residual esterase activity of the immobilized CALB compared with the free lipase depends directly on the amount of co‐adsorbed polyols. Moreover, polyols boost the catalytic performance in the kinetic resolution of racemic ibuprofen showing an optimum at the maximum coverage of polyols on the biocatalysts.
format Articulo
Articulo
author Llerena Suster, Carlos Rafael
Toledo, María Victoria
Fittipaldi, Antonela Soledad
Morcelle del Valle, Susana Raquel
Briand, Laura Estefanía
author_facet Llerena Suster, Carlos Rafael
Toledo, María Victoria
Fittipaldi, Antonela Soledad
Morcelle del Valle, Susana Raquel
Briand, Laura Estefanía
author_sort Llerena Suster, Carlos Rafael
title Lipase B of <i>Candida antarctica</i> co-adsorbed with polyols onto TiO<sub>2</sub> nanoparticles for improved biocatalytic performance
title_short Lipase B of <i>Candida antarctica</i> co-adsorbed with polyols onto TiO<sub>2</sub> nanoparticles for improved biocatalytic performance
title_full Lipase B of <i>Candida antarctica</i> co-adsorbed with polyols onto TiO<sub>2</sub> nanoparticles for improved biocatalytic performance
title_fullStr Lipase B of <i>Candida antarctica</i> co-adsorbed with polyols onto TiO<sub>2</sub> nanoparticles for improved biocatalytic performance
title_full_unstemmed Lipase B of <i>Candida antarctica</i> co-adsorbed with polyols onto TiO<sub>2</sub> nanoparticles for improved biocatalytic performance
title_sort lipase b of <i>candida antarctica</i> co-adsorbed with polyols onto tio<sub>2</sub> nanoparticles for improved biocatalytic performance
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103071
https://onlinelibrary.wiley.com/doi/10.1002/jctb.5305
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