Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)

In Argentina pseudorabies is an endemic disease. Routine diagnosis is made by virus isolation. It is a very long procedure to carry out and gives variable results depending on the quality of sample, hence the need for effective techniques, which are rapid and not dependent on the isolation of infect...

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Detalles Bibliográficos
Autores principales: Echeverría, María Gabriela, Pecoraro, Marcelo Ricardo Ítalo, Pereyra, N. B., Pidone, Claudio Luis, Galosi, Cecilia Mónica, Etcheverrigaray, María Elisa, Nosetto, Edgardo Omar
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Veterinarias
Diagnosis
Pseudorabies virus
Swine
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/102736
https://ri.conicet.gov.ar/11336/84848
https://www.aam.org.ar/ram.php?r=21
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-102736
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Diagnosis
Pseudorabies virus
Swine
spellingShingle Ciencias Veterinarias
Diagnosis
Pseudorabies virus
Swine
Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
Pereyra, N. B.
Pidone, Claudio Luis
Galosi, Cecilia Mónica
Etcheverrigaray, María Elisa
Nosetto, Edgardo Omar
Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
topic_facet Ciencias Veterinarias
Diagnosis
Pseudorabies virus
Swine
description In Argentina pseudorabies is an endemic disease. Routine diagnosis is made by virus isolation. It is a very long procedure to carry out and gives variable results depending on the quality of sample, hence the need for effective techniques, which are rapid and not dependent on the isolation of infectious virus. The polymerase chain reaction (PCR) technique has provided a sensitive, specific and rapid mean to detect DNA sequences. This study describes a PCR method for detection of pseudorabies virus sequences in swine tissues. In order to determine the presence of suid herpesvirus-1 DNA and antigens, 36 tissue samples collected from 19 dead pigs, with signs of pseudorabies infection, were examined by PCR, virus isolation and indirect immunofluorescence, respectively. Fifteen out of 19 pigs were positive at least for one tissue by PCR (15/19) while only three pseudorabies virus strains were isolated (3/19). All the amplified products were identified by digestion with Sa/l and hybridization. The method described herein circumvents tedious viral isolation and DNA purification and would be a valuable tool for rapid diagnosis, since it would take less than 5 h to reach an accurate result even in poorly preserved tissue samples.
format Articulo
Articulo
author Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
Pereyra, N. B.
Pidone, Claudio Luis
Galosi, Cecilia Mónica
Etcheverrigaray, María Elisa
Nosetto, Edgardo Omar
author_facet Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
Pereyra, N. B.
Pidone, Claudio Luis
Galosi, Cecilia Mónica
Etcheverrigaray, María Elisa
Nosetto, Edgardo Omar
author_sort Echeverría, María Gabriela
title Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_short Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_full Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_fullStr Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_full_unstemmed Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_sort rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (pcr)
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/102736
https://ri.conicet.gov.ar/11336/84848
https://www.aam.org.ar/ram.php?r=21
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