Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle

The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the nucleus of skeletal muscle cells where it hel...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Tan, Taylor, Jackson, Jiang, Yang, Pereyra, Andrea Soledad, Messi, Maria Laura, Wang, Zhong Min, Hereñú, Claudia Beatriz, Delbono, Osvaldo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Médicas
Biología
Cavβ1a
Nuclear localization
Skeletal muscle
Troponin T3
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/102639
https://ri.conicet.gov.ar/11336/48902
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522384/
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-102639
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Biología
Cavβ1a
Nuclear localization
Skeletal muscle
Troponin T3
spellingShingle Ciencias Médicas
Biología
Cavβ1a
Nuclear localization
Skeletal muscle
Troponin T3
Zhang, Tan
Taylor, Jackson
Jiang, Yang
Pereyra, Andrea Soledad
Messi, Maria Laura
Wang, Zhong Min
Hereñú, Claudia Beatriz
Delbono, Osvaldo
Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
topic_facet Ciencias Médicas
Biología
Cavβ1a
Nuclear localization
Skeletal muscle
Troponin T3
description The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160-244 aa) and Cavβ1a NH2-terminus (1-99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Cavβ1a/YFP shows that TnT3 facilitates Cavβ1a nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation.
format Articulo
Articulo
author Zhang, Tan
Taylor, Jackson
Jiang, Yang
Pereyra, Andrea Soledad
Messi, Maria Laura
Wang, Zhong Min
Hereñú, Claudia Beatriz
Delbono, Osvaldo
author_facet Zhang, Tan
Taylor, Jackson
Jiang, Yang
Pereyra, Andrea Soledad
Messi, Maria Laura
Wang, Zhong Min
Hereñú, Claudia Beatriz
Delbono, Osvaldo
author_sort Zhang, Tan
title Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_short Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_full Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_fullStr Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_full_unstemmed Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_sort troponin t3 regulates nuclear localization of the calcium channel cavβ1a subunit in skeletal muscle
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/102639
https://ri.conicet.gov.ar/11336/48902
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522384/
work_keys_str_mv AT zhangtan troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
AT taylorjackson troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
AT jiangyang troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
AT pereyraandreasoledad troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
AT messimarialaura troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
AT wangzhongmin troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
AT herenuclaudiabeatriz troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
AT delbonoosvaldo troponint3regulatesnuclearlocalizationofthecalciumchannelcavb1asubunitinskeletalmuscle
bdutipo_str Repositorios
_version_ 1764820441991479296

Ejemplares similares