Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)

This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak in a rearing facility and in wild H. gelotopoeon populations in Córdoba, Argentina. Ho...

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Detalles Bibliográficos
Autores principales:	Ferrelli, María Leticia, Martínez Taibo, Carolina, Fichetti, Patricia del Carmen, Sciocco Cap, Alicia, Arneodo Larochette, Joel Demián
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2016
Materias:	Biología Electron microscopy Helicoverpa gelotopoeon Nucleopolyhedrovirus Phylogenetic analysis
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/102060 https://ri.conicet.gov.ar/11336/53495
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-102060
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Electron microscopy Helicoverpa gelotopoeon Nucleopolyhedrovirus Phylogenetic analysis
spellingShingle	Biología Electron microscopy Helicoverpa gelotopoeon Nucleopolyhedrovirus Phylogenetic analysis Ferrelli, María Leticia Martínez Taibo, Carolina Fichetti, Patricia del Carmen Sciocco Cap, Alicia Arneodo Larochette, Joel Demián Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)
topic_facet	Biología Electron microscopy Helicoverpa gelotopoeon Nucleopolyhedrovirus Phylogenetic analysis
description	This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak in a rearing facility and in wild H. gelotopoeon populations in Córdoba, Argentina. Host identity was corroborated by partial sequencing of the COI gene. Scanning electron microscope observations of purified OBs revealed their polyhedral morphology and an average diameter of 0.89 ± 0.14 μm. Ultrathin sections of infected larvae examined by transmission electron microscopy showed the intranuclear occurrence of polyhedra and virus particles in fat body cells. Nucleocapsids were singly enveloped. Phylogenetic analysis of lef-8, lef-9, polh, orf5/5b and hr3-orf62 viral sequences identified this new NPV isolate (hereafter HegeSNPV) as a variant of Helicoverpa armigera nucleopolyhedrovirus (HearNPV). Furthermore, HegeSNPV was closely related to the so-called “HzSNPV Group” within HearNPV, although having particular characteristics.
format	Articulo Preprint
author	Ferrelli, María Leticia Martínez Taibo, Carolina Fichetti, Patricia del Carmen Sciocco Cap, Alicia Arneodo Larochette, Joel Demián
author_facet	Ferrelli, María Leticia Martínez Taibo, Carolina Fichetti, Patricia del Carmen Sciocco Cap, Alicia Arneodo Larochette, Joel Demián
author_sort	Ferrelli, María Leticia
title	Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)
title_short	Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)
title_full	Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)
title_fullStr	Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)
title_full_unstemmed	Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)
title_sort	characterization of a new <i>helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>helicoverpa gelotopoeon</i> (lepidoptera: noctuidae)
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/102060 https://ri.conicet.gov.ar/11336/53495
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Characterization of a new <i>Helicoverpa armigera nucleopolyhedrovirus</i> variant causing epizootic on a previously unreported host, <i>Helicoverpa gelotopoeon</i> (Lepidoptera: Noctuidae)

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