Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells

In the developing nervous system, neural stem cells are polarized and maintain an apical domain facing a central lumen. The presence of apical membrane is thought to have a profound influence on maintaining the stem cell state. With the onset of neurogenesis, cells lose their polarization, and the c...

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Autores principales:	Medelnik, Jan Philip, Roensch, Kathleen, Okawa, Satoshi, del Sol, Antonio, Chara, Osvaldo, Mchedlishvili, Levan, Tanaka, Elly M.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2018
Materias:	Biología Apical domain Cell polarization Human embryonic stem cells Lumen formation/organization Lysophosphatidic acid Neural progenitor cells Neural rosettes Neural stem cells Neuroepithelial cells
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/101966 https://ri.conicet.gov.ar/11336/93820
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-101966
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Apical domain Cell polarization Human embryonic stem cells Lumen formation/organization Lysophosphatidic acid Neural progenitor cells Neural rosettes Neural stem cells Neuroepithelial cells
spellingShingle	Biología Apical domain Cell polarization Human embryonic stem cells Lumen formation/organization Lysophosphatidic acid Neural progenitor cells Neural rosettes Neural stem cells Neuroepithelial cells Medelnik, Jan Philip Roensch, Kathleen Okawa, Satoshi del Sol, Antonio Chara, Osvaldo Mchedlishvili, Levan Tanaka, Elly M. Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells
topic_facet	Biología Apical domain Cell polarization Human embryonic stem cells Lumen formation/organization Lysophosphatidic acid Neural progenitor cells Neural rosettes Neural stem cells Neuroepithelial cells
description	In the developing nervous system, neural stem cells are polarized and maintain an apical domain facing a central lumen. The presence of apical membrane is thought to have a profound influence on maintaining the stem cell state. With the onset of neurogenesis, cells lose their polarization, and the concomitant loss of the apical domain coincides with a loss of the stem cell identity. Little is known about the molecular signals controlling apical membrane size. Here, we use two neuroepithelial cell systems, one derived from regenerating axolotl spinal cord and the other from human embryonic stem cells, to identify a molecular signaling pathway initiated by lysophosphatidic acid that controls apical membrane size and consequently controls and maintains epithelial organization and lumen size in neuroepithelial rosettes. This apical domain size increase occurs independently of effects on proliferation and involves a serum response factor-dependent transcriptional induction of junctional and apical membrane components. Medelnik and colleagues identify lysophosphatidic acid as a serum factor that has a major effect on the expansion of the apical domain of human ESC-derived neural progenitor cells, resulting in the formation of very large neural rosette-like structures that can be maintained for many passages in the absence of neuronal and glial differentiation if constantly supplied with LPA.
format	Articulo Articulo
author	Medelnik, Jan Philip Roensch, Kathleen Okawa, Satoshi del Sol, Antonio Chara, Osvaldo Mchedlishvili, Levan Tanaka, Elly M.
author_facet	Medelnik, Jan Philip Roensch, Kathleen Okawa, Satoshi del Sol, Antonio Chara, Osvaldo Mchedlishvili, Levan Tanaka, Elly M.
author_sort	Medelnik, Jan Philip
title	Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells
title_short	Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells
title_full	Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells
title_fullStr	Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells
title_full_unstemmed	Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells
title_sort	signaling-dependent control of apical membrane size and self-renewal in rosette-stage human neuroepithelial stem cells
publishDate	2018
url	http://sedici.unlp.edu.ar/handle/10915/101966 https://ri.conicet.gov.ar/11336/93820
work_keys_str_mv	AT medelnikjanphilip signalingdependentcontrolofapicalmembranesizeandselfrenewalinrosettestagehumanneuroepithelialstemcells AT roenschkathleen signalingdependentcontrolofapicalmembranesizeandselfrenewalinrosettestagehumanneuroepithelialstemcells AT okawasatoshi signalingdependentcontrolofapicalmembranesizeandselfrenewalinrosettestagehumanneuroepithelialstemcells AT delsolantonio signalingdependentcontrolofapicalmembranesizeandselfrenewalinrosettestagehumanneuroepithelialstemcells AT charaosvaldo signalingdependentcontrolofapicalmembranesizeandselfrenewalinrosettestagehumanneuroepithelialstemcells AT mchedlishvililevan signalingdependentcontrolofapicalmembranesizeandselfrenewalinrosettestagehumanneuroepithelialstemcells AT tanakaellym signalingdependentcontrolofapicalmembranesizeandselfrenewalinrosettestagehumanneuroepithelialstemcells
bdutipo_str	Repositorios
_version_	1764820440616796162

Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells

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