Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation

Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free...

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Autores principales: La Greca, Alejandro Damián, Solari, Claudia María, Furmento, Verónica Alejandra, Lombardi, Antonella, Biani, María Celeste, Aban, Cyntia Estefanía, Moro, Lucía Natalia, García, Marcela Nilda, Guberman, Alejandra Sonia, Sevlever, Gustavo, Miriuka, Santiago Gabriel, Luzzani, Carlos Daniel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
Extracellular vesicles
Mesenchymal stem cells
Proteomic
Differentiation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101459
https://ri.conicet.gov.ar/11336/93881
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-101459
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Extracellular vesicles
Mesenchymal stem cells
Proteomic
Differentiation
spellingShingle Ciencias Exactas
Extracellular vesicles
Mesenchymal stem cells
Proteomic
Differentiation
La Greca, Alejandro Damián
Solari, Claudia María
Furmento, Verónica Alejandra
Lombardi, Antonella
Biani, María Celeste
Aban, Cyntia Estefanía
Moro, Lucía Natalia
García, Marcela Nilda
Guberman, Alejandra Sonia
Sevlever, Gustavo
Miriuka, Santiago Gabriel
Luzzani, Carlos Daniel
Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
topic_facet Ciencias Exactas
Extracellular vesicles
Mesenchymal stem cells
Proteomic
Differentiation
description Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced in large quantities and stored until ready to use. Although the therapeutic potential of MSC EVs has already been proven, a thorough characterization of MSC EVs is lacking. In this work, we used a label-free liquid chromatography tandem mass spectrometry proteomic approach to identify the most abundant proteins in EVs that are secreted from MSCs derived from PSCs (PD-MSCs) and from their parental induced PSCs (iPSCs). Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell–substrate adhesion. Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. Correlation and principal component analysis consistently aggregate iPSCs and iPSC EVs but segregate PD-MSC and their EVs. Altogether, these findings suggest that during differentiation, compared with their parental iPSC EVs, PD-MSC EVs acquire a more specific set of proteins; arguably, this difference might confer their therapeutic properties.
format Articulo
Articulo
author La Greca, Alejandro Damián
Solari, Claudia María
Furmento, Verónica Alejandra
Lombardi, Antonella
Biani, María Celeste
Aban, Cyntia Estefanía
Moro, Lucía Natalia
García, Marcela Nilda
Guberman, Alejandra Sonia
Sevlever, Gustavo
Miriuka, Santiago Gabriel
Luzzani, Carlos Daniel
author_facet La Greca, Alejandro Damián
Solari, Claudia María
Furmento, Verónica Alejandra
Lombardi, Antonella
Biani, María Celeste
Aban, Cyntia Estefanía
Moro, Lucía Natalia
García, Marcela Nilda
Guberman, Alejandra Sonia
Sevlever, Gustavo
Miriuka, Santiago Gabriel
Luzzani, Carlos Daniel
author_sort La Greca, Alejandro Damián
title Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
title_short Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
title_full Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
title_fullStr Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
title_full_unstemmed Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
title_sort extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/101459
https://ri.conicet.gov.ar/11336/93881
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