Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
A breakdown in intestinal homeostasis results in inflammatory bowel diseases including coeliac disease and allergy. Galectins, evolutionarily conserved beta-galactoside-binding proteins, can modulate immune-epithelial cell interactions by influencing immune cell fate and cytokine secretion. In this...
Guardado en:
| Autores principales: | , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2009
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/101008 https://ri.conicet.gov.ar/11336/25863 http://journals.sagepub.com/doi/abs/10.1177/039463200902200123 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844523/ http://journals.sagepub.com/doi/abs/10.1177/039463200902200123 |
| Aporte de: |
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I19-R120-10915-101008 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica y biología molecular Medicina básica Ciencias médicas y de la salud Gut Lymphocytes Galectin-3 Allergy |
| spellingShingle |
Bioquímica y biología molecular Medicina básica Ciencias médicas y de la salud Gut Lymphocytes Galectin-3 Allergy Mercer, Natalia Guzman, Luciana Cueto Rua, Eduardo Drut, Ricardo Ahmed, H. Vasta, G. R. Toscano, Marta Alicia Rabinovich, Gabriel Adrián Docena, Guillermo Horacio Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3 |
| topic_facet |
Bioquímica y biología molecular Medicina básica Ciencias médicas y de la salud Gut Lymphocytes Galectin-3 Allergy |
| description |
A breakdown in intestinal homeostasis results in inflammatory bowel diseases including coeliac disease and allergy. Galectins, evolutionarily conserved beta-galactoside-binding proteins, can modulate immune-epithelial cell interactions by influencing immune cell fate and cytokine secretion. In this study we investigated the glycosylation signature, as well as the regulated expression of galectin-1 and -3 in human duodenal samples of allergic and non-allergic children. Whereas galectin-1 was predominantly localized in the epithelial compartment (epithelial cells and intraepithelial lymphocytes) and the underlying lamina propria (T cells, macrophages and plasma cells), galectin-3 was mainly expressed by crypt epithelial cells and macrophages in the lamina propria. Remarkably, expression of these galectins was not significantly altered in allergic versus non-allergic patients. Investigation of the glycophenotype of the duodenal inflammatory microenvironment revealed substantial alpha2-6-linked sialic acid bound to galactose in lamina propria plasma cells, macrophages and intraepithelial lymphocytes and significant levels of asialo core 1 O-glycans in CD68+ macrophages and enterocytes. Galectin-1 preferentially bound to neutrophils, plasma cells and enterocytes, while galectin-3 binding sites were mainly distributed on macrophages and intraepithelial lymphocytes. Notably, galectin-3, but not galectin-1 binding, was substantially increased in intraepithelial gut lymphocytes of allergic patients compared to non-allergic subjects, suggesting a potential role of galectin-3-glycan interactions in shaping epithelial-immune cell connections during allergic inflammatory processes. |
| format |
Articulo Articulo |
| author |
Mercer, Natalia Guzman, Luciana Cueto Rua, Eduardo Drut, Ricardo Ahmed, H. Vasta, G. R. Toscano, Marta Alicia Rabinovich, Gabriel Adrián Docena, Guillermo Horacio |
| author_facet |
Mercer, Natalia Guzman, Luciana Cueto Rua, Eduardo Drut, Ricardo Ahmed, H. Vasta, G. R. Toscano, Marta Alicia Rabinovich, Gabriel Adrián Docena, Guillermo Horacio |
| author_sort |
Mercer, Natalia |
| title |
Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3 |
| title_short |
Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3 |
| title_full |
Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3 |
| title_fullStr |
Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3 |
| title_full_unstemmed |
Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3 |
| title_sort |
duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3 |
| publishDate |
2009 |
| url |
http://sedici.unlp.edu.ar/handle/10915/101008 https://ri.conicet.gov.ar/11336/25863 http://journals.sagepub.com/doi/abs/10.1177/039463200902200123 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844523/ http://journals.sagepub.com/doi/abs/10.1177/039463200902200123 |
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