Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>

This study investigated the structural and biophysical characteristics of GumB and GumC, two Xanthomonas campestris membrane proteins that are involved in xanthan biosynthesis. Xanthan is an exopolysaccharide that is thought to be a virulence factor that contributes to bacterial in planta growth. It...

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Detalles Bibliográficos
Autores principales:	Bianco, María Isabel, Jacobs, Melisa, Salinas, Silvina Rosa, Salvay, Andrés Gerardo, Ielmini, M. V., Ielpi, Luis
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2014
Materias:	Ciencias Exactas Exopolysaccharide Membrane proteins Xanthan Xanthomonas campestris
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/100971 https://ri.conicet.gov.ar/11336/24988
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-100971
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Exopolysaccharide Membrane proteins Xanthan Xanthomonas campestris
spellingShingle	Ciencias Exactas Exopolysaccharide Membrane proteins Xanthan Xanthomonas campestris Bianco, María Isabel Jacobs, Melisa Salinas, Silvina Rosa Salvay, Andrés Gerardo Ielmini, M. V. Ielpi, Luis Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>
topic_facet	Ciencias Exactas Exopolysaccharide Membrane proteins Xanthan Xanthomonas campestris
description	This study investigated the structural and biophysical characteristics of GumB and GumC, two Xanthomonas campestris membrane proteins that are involved in xanthan biosynthesis. Xanthan is an exopolysaccharide that is thought to be a virulence factor that contributes to bacterial in planta growth. It also is one of the most important industrial biopolymers. The first steps of xanthan biosynthesis are well understood, but the polymerization and export mechanisms remain unclear. For this reason, the key proteins must be characterized to better understand these processes. Here we characterized, by biochemical and biophysical techniques, GumB, the outer membrane polysaccharide export protein, and GumC, the polysaccharide co-polymerase protein of the xanthan biosynthesis system. Our results suggested that recombinant GumB is a tetrameric protein in solution. On the other hand, we observed that both native and recombinant GumC present oligomeric conformation consistent with dimers and higher-order oligomers. The transmembrane segments of GumC are required for GumC expression and/or stability. These initial results provide a starting point for additional studies that will clarify the roles of GumB and GumC in the xanthan polymerization and export processes and further elucidate their functions and mechanisms of action.
format	Articulo Preprint
author	Bianco, María Isabel Jacobs, Melisa Salinas, Silvina Rosa Salvay, Andrés Gerardo Ielmini, M. V. Ielpi, Luis
author_facet	Bianco, María Isabel Jacobs, Melisa Salinas, Silvina Rosa Salvay, Andrés Gerardo Ielmini, M. V. Ielpi, Luis
author_sort	Bianco, María Isabel
title	Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>
title_short	Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>
title_full	Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>
title_fullStr	Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>
title_full_unstemmed	Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>
title_sort	biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>xanthomonas campestris</i>
publishDate	2014
url	http://sedici.unlp.edu.ar/handle/10915/100971 https://ri.conicet.gov.ar/11336/24988
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Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from <i>Xanthomonas campestris</i>

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