Advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-I collagen matrix
The adhesion of osteoblasts to bone extracellular matrix, of which type-I collagen constitutes >85%, can modulate diverse aspects of their physiology such as growth, differentiation and mineralisation. In this study we examined the adhesion of UMR106 rat osteoblast-like cells either to a control...
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Formato: | Articulo |
Lenguaje: | Inglés |
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2004
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/100327 |
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I19-R120-10915-100327 |
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Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
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SEDICI (UNLP) |
language |
Inglés |
topic |
Química Advanced glycation endproducts Osteoblast Adhesion Integrin receptors Type-I collagen |
spellingShingle |
Química Advanced glycation endproducts Osteoblast Adhesion Integrin receptors Type-I collagen McCarthy, Antonio Desmond Uemura, Toshimasa Etcheverry, Susana Beatriz Cortizo, Ana María Advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-I collagen matrix |
topic_facet |
Química Advanced glycation endproducts Osteoblast Adhesion Integrin receptors Type-I collagen |
description |
The adhesion of osteoblasts to bone extracellular matrix, of which type-I collagen constitutes >85%, can modulate diverse aspects of their physiology such as growth, differentiation and mineralisation. In this study we examined the adhesion of UMR106 rat osteoblast-like cells either to a control (Col) or advanced-glycation-endproduct-modified (AGEs-Col) type I collagen matrix. We investigated the possible role of different integrin receptors in osteoblastic adhesion, by co-incubating these cells either with β-peptide (conserved sequence 113–125 of the β subunit of integrins) or with two other peptides, RGD (Arg-Gly-Asp) and DGEA (Asp-Gly-Glu-Ala), which are recognition sequences for the α-subunits of α1,5β1and α2β1integrins. Collagen glycation inhibited the adhesion of UMR106 osteoblasts to the matrix (40% reduction versus Col,P<0.001). β-Peptide showed a dose- and glycation-dependent inhibitory effect on adhesion, and at a concentration of 100μM decreased the attachment of UMR106 cells to both matrices (42% to Col,P<0.001; and 25% to AGEs-Col,P<0.01). The synthetic peptides RGD (1mM) and DGEA (5mM) inhibited the attachment of UMR106 cells to Col (30 and 20%,P<0.01 andP<0.001, respectively), but not to AGEs-Col. β-Peptide induced an increase in UMR106 cell clumping and a decrease in cellular spreading, while DGEA increased spreading with cellular extensions in multiple directions. These results indicate that both α and β integrin subunits participate in osteoblastic attachment to type-I collagen, probably through the α1,5β1and α2β1integrins. AGEs-modification of type-I collagen impairs the integrin-mediated adhesion of osteoblastic cells to the matrix, and could thus contribute to the pathogenesis of diabetic osteopenia. |
format |
Articulo Articulo |
author |
McCarthy, Antonio Desmond Uemura, Toshimasa Etcheverry, Susana Beatriz Cortizo, Ana María |
author_facet |
McCarthy, Antonio Desmond Uemura, Toshimasa Etcheverry, Susana Beatriz Cortizo, Ana María |
author_sort |
McCarthy, Antonio Desmond |
title |
Advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-I collagen matrix |
title_short |
Advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-I collagen matrix |
title_full |
Advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-I collagen matrix |
title_fullStr |
Advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-I collagen matrix |
title_full_unstemmed |
Advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-I collagen matrix |
title_sort |
advanced glycation endproducts interfere with integrin-mediated osteoblastic attachment to a type-i collagen matrix |
publishDate |
2004 |
url |
http://sedici.unlp.edu.ar/handle/10915/100327 |
work_keys_str_mv |
AT mccarthyantoniodesmond advancedglycationendproductsinterferewithintegrinmediatedosteoblasticattachmenttoatypeicollagenmatrix AT uemuratoshimasa advancedglycationendproductsinterferewithintegrinmediatedosteoblasticattachmenttoatypeicollagenmatrix AT etcheverrysusanabeatriz advancedglycationendproductsinterferewithintegrinmediatedosteoblasticattachmenttoatypeicollagenmatrix AT cortizoanamaria advancedglycationendproductsinterferewithintegrinmediatedosteoblasticattachmenttoatypeicollagenmatrix |
bdutipo_str |
Repositorios |
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1764820440206802947 |