Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions

Enterococcus is able to grow in media at pH from 5.0 to 9.0 and a high concentration of NaCl (8%). The ability to respond to these extreme conditions requires the rapid movement of three critical ions: proton (H+), sodium (Na+), and potassium (K+). The activity of the proton F0F1 ATPase and the s...

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Autores principales: Acciarri, Giuliana, Gizzi, Fernán O., Torres Manno, Mariano, Stülke, Jörg, Blancato, Victor, Magni, Christian
Lenguaje:Inglés
Publicado: Frontiers Media S.A. 2023
Materias:
Enterococcus faecalis
Potassium transport
KUP/HAK/KT K+ transporters
Ktr family
Kdp system
Acceso en línea:http://hdl.handle.net/2133/25721
http://hdl.handle.net/2133/25721
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-25721
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Enterococcus faecalis
Potassium transport
KUP/HAK/KT K+ transporters
Ktr family
Kdp system
spellingShingle Enterococcus faecalis
Potassium transport
KUP/HAK/KT K+ transporters
Ktr family
Kdp system
Acciarri, Giuliana
Gizzi, Fernán O.
Torres Manno, Mariano
Stülke, Jörg
Blancato, Victor
Magni, Christian
Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions
topic_facet Enterococcus faecalis
Potassium transport
KUP/HAK/KT K+ transporters
Ktr family
Kdp system
description Enterococcus is able to grow in media at pH from 5.0 to 9.0 and a high concentration of NaCl (8%). The ability to respond to these extreme conditions requires the rapid movement of three critical ions: proton (H+), sodium (Na+), and potassium (K+). The activity of the proton F0F1 ATPase and the sodium Na+ V0V1 type ATPase under acidic or alkaline conditions, respectively, is well established in these microorganisms. The potassium uptake transporters KtrI and KtrII were described in Enterococcus hirae, which were associated with growth in acidic and alkaline conditions, respectively. In Enterococcus faecalis, the presence of the Kdp (potassium ATPase) system was early established. However, the homeostasis of potassium in this microorganism is not completely explored. In this study, we demonstrate that Kup and KimA are highaffinity potassium transporters, and the inactivation of these genes in E. faecalis JH2-2 (a Kdp laboratory natural deficient strain) had no effect on the growth parameters. However, in KtrA defective strains (ΔktrA, ΔkupΔktrA) an impaired growth was observed under stress conditions, which was restored to wild type levels by external addition of K+ ions. Among the multiplicity of potassium transporters identify in the genus Enterococcus, Ktr channels (KtrAB and KtrAD), and Kup family symporters (Kup and KimA) are present and may contribute to the particular resistance of these microorganisms to different stress conditions. In addition, we found that the presence of the Kdp system in E. faecalis is strain-dependent, and this transporter is enriched in strains of clinical origin as compared to environmental, commensal, or food isolates.
author Acciarri, Giuliana
Gizzi, Fernán O.
Torres Manno, Mariano
Stülke, Jörg
Blancato, Victor
Magni, Christian
author_facet Acciarri, Giuliana
Gizzi, Fernán O.
Torres Manno, Mariano
Stülke, Jörg
Blancato, Victor
Magni, Christian
author_sort Acciarri, Giuliana
title Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions
title_short Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions
title_full Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions
title_fullStr Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions
title_full_unstemmed Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions
title_sort redundant potassium transporter systems guarantee the survival of enterococcus faecalis under stress conditions
publisher Frontiers Media S.A.
publishDate 2023
url http://hdl.handle.net/2133/25721
http://hdl.handle.net/2133/25721
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AT gizzifernano redundantpotassiumtransportersystemsguaranteethesurvivalofenterococcusfaecalisunderstressconditions
AT torresmannomariano redundantpotassiumtransportersystemsguaranteethesurvivalofenterococcusfaecalisunderstressconditions
AT stulkejorg redundantpotassiumtransportersystemsguaranteethesurvivalofenterococcusfaecalisunderstressconditions
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spelling I15-R121-2133-257212023-05-22T14:34:12Z Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions Acciarri, Giuliana Gizzi, Fernán O. Torres Manno, Mariano Stülke, Jörg Blancato, Victor Magni, Christian Enterococcus faecalis Potassium transport KUP/HAK/KT K+ transporters Ktr family Kdp system Enterococcus is able to grow in media at pH from 5.0 to 9.0 and a high concentration of NaCl (8%). The ability to respond to these extreme conditions requires the rapid movement of three critical ions: proton (H+), sodium (Na+), and potassium (K+). The activity of the proton F0F1 ATPase and the sodium Na+ V0V1 type ATPase under acidic or alkaline conditions, respectively, is well established in these microorganisms. The potassium uptake transporters KtrI and KtrII were described in Enterococcus hirae, which were associated with growth in acidic and alkaline conditions, respectively. In Enterococcus faecalis, the presence of the Kdp (potassium ATPase) system was early established. However, the homeostasis of potassium in this microorganism is not completely explored. In this study, we demonstrate that Kup and KimA are highaffinity potassium transporters, and the inactivation of these genes in E. faecalis JH2-2 (a Kdp laboratory natural deficient strain) had no effect on the growth parameters. However, in KtrA defective strains (ΔktrA, ΔkupΔktrA) an impaired growth was observed under stress conditions, which was restored to wild type levels by external addition of K+ ions. Among the multiplicity of potassium transporters identify in the genus Enterococcus, Ktr channels (KtrAB and KtrAD), and Kup family symporters (Kup and KimA) are present and may contribute to the particular resistance of these microorganisms to different stress conditions. In addition, we found that the presence of the Kdp system in E. faecalis is strain-dependent, and this transporter is enriched in strains of clinical origin as compared to environmental, commensal, or food isolates. Fil: Acciarri, Giuliana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Fisiología y Genética de Bacterias Lácticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. Fil: Gizzi, Fernán O. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Fisiología y Genética de Bacterias Lácticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. Fil: Torres Manno, Mariano. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Fisiología y Genética de Bacterias Lácticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. Fil: Blancato, Victor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Fisiología y Genética de Bacterias Lácticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. Fil: Magni, Christian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Fisiología y Genética de Bacterias Lácticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. Fil: Torres Manno, Mariano. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Bioinformática. Departamento de Matemática y Estadística; Argentina. Fil: Stülke, Jörg. Georg August University. Department of General Microbiology; Germany. Fil: Blancato, Victor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Biotecnología e Inocuidad de los Alimentos. Área de Biotecnología de los Alimentos; Argentina. Fil: Magni, Christian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Biotecnología e Inocuidad de los Alimentos. Área de Biotecnología de los Alimentos; Argentina. 2023-05-22T14:22:40Z 2023-05-22T14:22:40Z 2023-02-08 2023-05-22T14:22:40Z 2023-05-22T14:22:40Z 2023-02-08 1664-302X http://hdl.handle.net/2133/25721 http://hdl.handle.net/2133/25721 eng https://doi.org/10.3389/fmicb.2023.1117684 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1117684/full http://creativecommons.org/licenses/by/2.5/ar/ Acciarri, Giuliana Gizzi, Fernán O. Torres Manno, Mariano Stülke, Jörg Blancato, Victor Magni, Christian      Attribution 4.0 International (CC BY 4.0) openAccess application/pdf Frontiers Media S.A.

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